慢性铅暴露对小鼠海马PKC-γ蛋白表达影响

Effect of chronic lead exposure on expression of PKC-γ in mice hippocampus

摘要: 目的观察铅对小鼠海马蛋白激酶C中γ亚型(PKC-γ)蛋白表达的影响。方法幼鼠出生第1 d起,染铅组母鼠开始通过饮水饲以不同浓度醋酸铅2.4,4.8,9.6 mmol/L。幼鼠出生后,先通过哺乳接触铅,断乳后则自行饮用与母鼠饮用浓度相同的含铅水。分别在第14,21,35,48 d处死小鼠,用蛋白免疫印记法检测各组小鼠海马区PKC-γ蛋白表达状况。结果慢性铅露对小鼠脑海马PKC-γ蛋白表达总体上呈现下降趋势,各染铅组中,PKC-γ蛋白表达与相应对照组比较,差异均有统计学意义(P<0.05)。结论铅扰乱PKC-γ蛋白正常表达。

Abstract: ObjectiveTo observe the effect of chronic lead contamination on protein expression of PKC-C in the hippocampus of mice.MethodsChronic acetic lead contamination was applied to mice(2.4,4.8,9.6 mmol/L),and young mice were killed at 14,21,35,48 d respectively.Western blotting was used to observe the protein expression levels of PKC-C in hippocampus of mice.ResultsCompared with the control group,PKC-Cprotein expression in the hippocampus of mice with chronic lead exposure was significantly down trended(P<0.05).ConclusionChronic lead exposure can disturb the normal expression of PKC-C protein in mice.