Abstract: Objective To explore the effect of cola on the quality and quantity of spermatozoa of male mice.Methods 31 male mice of Kunming strain were divided randomly into 5 groups:negative control group treated with normal saline 0.6 ml;low-dose group with 0.3 ml of cola,high-dose group with 0.6 ml of cola;abdominal injection group with 0.6 ml of cola and positive control group with 0.6 ml of cytox an once daily for 7 days.Then,the mice were killed and their epididymis were collected and the movement parameter and deformity of the sperm were obser ved.Results The kidney coefficient of high dosage group had the significance difference compared with positive control group and low dosage group.The epididymis coefficient of abdominal injection group was significantly higher than that of positive control group(P<0.05).HB,PLT and WBC of every group was lower than positive control group.MAD of abdominal injection group was significantly higher than positive control group.The "ρ" of low dosage group had the significance difference compared with positive control group(P<0.05).The sperm deformity rates of low dosage group,high dosage group and negative control group were significantly higher than that of positive control group(P<0.05).The spermdeformity rates of low dosage group,high dosage group and abdominal injection group had the significance difference compared with negative control group(P<0.05).The sperm deformity rates of low dosage group and high-dosage group had the significance difference compared with abdominal injection group (P<0.05).Conclusion Cola has certain effect on spermatozoa activeness and its forms of male mice.