铅和镉对雄性小鼠生殖细胞DNA损伤的研究

Study on DAN damage of germ cells of mice treated with plumbum(Ⅱ) or cadmium(Ⅱ)

摘要:
目的研究一定剂量的醋酸铅、氯化镉对体外培养的雄性小鼠生殖细胞DNA损伤的作用。
方法应用单细胞凝胶电泳技术检测不同浓度铅、镉离子对雄性小鼠生殖细胞DNA损伤。
结果不同浓度的醋酸铅、氯化镉均可引起小鼠生殖细胞DNA的损伤, 并存在明显的剂量-反应关系。
结论体外条件下, 醋酸铅、氯化镉可引起雄性小鼠生殖细胞DNA的损伤。

Abstract:
Objective To study the effects of plumbum(Ⅱ) or cadmium(Ⅱ) on DNA damage of germ cells of male mice in vitro.
Methods The DNA damage was observed by means of SCGE assay.
Results Different dose plumbum(Ⅱ) or cadmium(Ⅱ) could cause DNA damages in male mice germ cells and there was significant dose-effect relationship.
Conclusion Plumbum(Ⅱ) or cadmium(Ⅱ) could cause DNA damage of germ cells in vitro.