Abstract: Objective To evaluate levels of job burnout and their associations with mental health among medical staff in hospitals and health personnel in centers for disease control and prevention (CDC).Methods Maslach Burnout Inventory-General Survey (MBI-GS) was self-administered to evaluate the job burnout level and Kessler Mental Health Questionnaire (K10) to evaluate the mental health among medical practitioners and nurses in hospitals at municipality or township level,and public health workers in CDCs at municipality and county level selected with multistage cluster random sampling in Liaoning province.Multiple hierarchical regression analysis was applied to elucidate correlation of job burnout and mental health among CDC workers,medical practitioners and nurses.Results The mean score of three subscales of burnout,including emotional exhaustion,cynicism and personal accomplishment,were 2.23±1.37,1.37±1.33,and 3.83±1.42,respectively.The mean score of mental health was 20.24±8.07.There was no significant difference in the prevalence of job burnout between CDC workers and medical practitioners.The prevalence of burnout in the nurses was significantly higher than that of the CDC workers and medical practitioners.Job burnout had significantly predictive effect on mental health.Conclusion Job burnout and mental health problems among medical personnel in Liaoning province are serious and positive attitude and effective measures should be promoted to alleviate job burnout and to prevent mental health problems among the populations.