Abstract: Objective To access the situation of drug-resistance tuberculosis in Fujian province and to provide evidence for making TB control policy.Methods All new registered sputum smear-positive patients in 9 surveillance sites of Fujian province were collected from September 2008 to August 2009.Identification and susceptibility tests were done for all culture positive samples.Proportion method was used to conduct drug sensitivity test of isonizid,rifampicin,streptomycin,and ethambutol according to the guidelines for surveillance of drug resistance(DR) in tuberculosis of WHO/IUATLD.Results Totally 1 014 TB cases were enrolled.The total DR rate was 19.8% and total multi-drug resistance(MDR) rate was 4.6%.The initial DR rate was 17.9% and initial MDR rate was 3.4%.The acquired DR rate was 44.0% and acquired MDR rate was 20.0%.Conclusion The prevalence of drug-resistant tuberculosis in Fujian province was lower than that of the average national level but higher than average international level,suggesting the necessity to strengthen the DR tuberculosis control program.