Abstract: Objective To examine vaccination rate of hepatitis B virus（ HBV） vaccine and its related factors,knowledge rate,and attitude in migrant workers and to provide evidence for health education in the population. Methods A questionnaire survey on rate of vaccination,awareness and attitude towards HBV was conducted among 1 447 migrant workers aged more than 20 years from enterprises in Zhangjiagang,Danyang and Taixing city. Logistic regression was used in statistic analysis. Results The hepatitis B vaccination rate was 59. 8%（ 95% confidence interval95% CI:57. 3%- 62. 4%）. The vaccination rate was associated with age,marriage status,educational level,living condition,and whether with health education（ P ＜ 0. 05 for all）. Logistic analyses showed that hepatitis B prevention knowledge（ odds ratioOR= 5. 573）,health education（ OR = 2. 811）,education level（ OR = 0. 2002）,and age（ OR = 0. 486） were positively correlated with vaccination behavior. The general awareness rate of HBV infection among the worker was 68. 5%.Main reasons for without HBV vaccination were not knowing the institution for the vaccination（ 29%）,worrying about side-effect（ 19. 8%）,and suspecting the efficacy of the vaccine（ 17. 7%）. There were 13. 2% of the workers reporting the discrimination to hepatitis B patients / carriers. Conclusion Migrant workers are key population of HBV vaccination and health education on HBV vaccination should be improved to increase the vaccination rate in the population.