Abstract: Objective To observe the effect of mulberry flavone on oxidative stress in type 2 diabetes rats with insulin resistance and to explore molecular mechanism of mulberry flavone against insulin resistance.Methods The adult male SD rats were fed with high-fat diet for 8 weeks,and then randomly divided into model group with type 2 diabetes(MOD group),mulberry leaves flavone treatment group(MLF),and normal control group(NOR group).We observed levels of fasting plasma glucose(FPG),fasting insulin(FINS),serum malondialdehyde(MDA),superoxide dismutase(SOD),glutathione peroxidase(GSH-Px)activity,insulin sensitivity index(ISI),and insulin resistance index(IRI).Results Compared with the normal group,serum MDA(8.73±0.85 nmol/L)was significantly increased;SOD(226.45±17.54 U/ml)and GSH-Px(529.03±69.56 U/ml)activity were significantly lower in model group.Compared with model group,mulberry flavone could significantly reduce FINS(25.83±2.1 mU/L)and IRI(8.86±0.96),increase ISI(5.08±0.09),lower FPG(7.95±1.85 mmol/L),and increase SOD(297.85±22.16 U/ml)activity,decrease MDA(6.82±0.79 nmol/ml) content.Conclusion Mulberry flavone can improve insulin resistance in type 2 diabetic rats with hyperinsulinemia,lower plasma glucose,improve insulin sensitivity,and resist oxidative stress.