Abstract: Objective To investigate the variation and distribution of microorganism on the surface of microphone head.Methods The specimens were collected with sterilized sw ab scrubbing.The abundance and density of the microorganisms were detected with bacterial culture and identification tests.The antigen of respiratory virus was detected with direct fluorescence antibody(DFA)and the specific DNA of Mycoplasma pneumoniae,Chlamydiae trachomatis,and Chlamydophila pneumoniae were detected with polymerase chain reaction(PCR).Results There were 3-9 bacterial species belonging to 2-6 genuses on the surface of microphone head.The positive detection rates of Staphylococcus,Streptococcus,Neisseria,Corynebacterium,and Bacillus were 17.33%-59.77%,21.21%-88.89%,15.78%-23.54%, 5.67%-11.12%,and 2.08%-12.36%,respectively.The microorganisms detected were mainly normal bacteria of oral cavity and pharyngonasal cavity,as well as the common bacteria in the environment.The antigen positive rate was 56.25%, 87.50%,68.75%,81.25%,43.75%,43.75%,and 68.75% for adenovirus,respiratory syncytial virus,influenza virus A, influenza virus B,parainfluenza virus 1,parainfluenza virus 2 and parainfluenza virus 3,respectively.The positive rate of Chlamydiae trachomatis was 6.25%.Mycoplasma pneumoniae and Chlamydophila pneumoniae were not checked out.Conclusion There were abundant microbial populations on the surface of microphone head,including pathogen and conditioned pathogen.The microphone is a potential vector associated with respiratory infections.