17β雌二醇对脂多糖致炎性拮抗作用

张莉莉; 解博红; 郭继强; 宋向凤

doi:10.11847/zgggws-2012-28-02-26

17β雌二醇对脂多糖致炎性拮抗作用

doi: 10.11847/zgggws-2012-28-02-26

1.
新乡医学院三全学院微生物学教研室, 河南新乡453003;
2.
新乡医学院免疫学教研室

基金项目:

河南省教育厅立项课题(200510472009)

详细信息

作者简介:
张莉莉(1984年- ),女,河南睢县人,助教,学士,研究方向:神经内分泌免疫。

通信作者:
宋向凤,E-mail:xfsong@xxmu.edu.cn

中图分类号: R392.6
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- 文章访问数: 900
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- 被引次数: 0
出版历程
- 接收日期: 2010-10-19
- 刊出日期: 2012-02-10

Effects of 17β-estradiol on expression of NF-кB and TLR4 in LPS-stimulated RAW264.7 cells

1.
Department of Microbiology, Sanquan College, Xinxiang Medical University, Xinxiang 453003, China

摘要

摘要: 目的研究17β雌二醇(17β-estradiol,E2)对核转录因子(NF-кB)转录活性及Toll样受体(TLR4)表达的影响。方法采用脂多糖和17β雌二醇同时作用于单核巨噬细胞RAW264.7,采用逆转录-聚合酶链反应方法检测细胞表面TLR4基因的表达;用荧光素酶报告基因转染试验观察NF-кB转录活性变化。结果对照组、LPS单独刺激组和17β雌二醇预作用组细胞TLR4 mRNA的相对表达分别为(1.23±0.21)、(1.52±0.28)和(1.11±0.27),NF-κB转录活性的相对值分别为(126±32)、(306±58)和(247±37),LPS刺激可使细胞TLR4 mRNA表达和NF-кB转录活性增强,而17β雌二醇预作用可明显降低TLR4 mRNA的表达和NF-кB转录活性,组间比较差异具有统计学意义(P<0.05)。结论 17β雌二醇能通过调节TLR4及转录因子NF-кB的活性呈现一定的抗炎作用。
- 雌激素 /
- 巨噬细胞 /
- 核转录因子(NF-кB) /
- Toll样受体(TLR4)
Abstract: Objective To investigate the effects of 17β-estradiol on the expression of nuclear factor kappa B(NF-кB) and Toll-like receptor 4(TLR4)in lipoplysaccharide(LPS)-stimulated RAW264.7 cells. Methods The expression of TLR4 mRNA in RAW264.7 cells was detected with reverse-tanscription-PCR(RT-PCR).The activity of transcription factor NF-кB was detected with method of luciferase reporter. Results The relative expression level of TLR4 mRNA was 1.23±0.21, 1.52±0.28,and 1.11±0.27 in the control group,LPS stimulation group,and 17β-estradiol group,respectively.Meanwhile, the relative value of NF-кB activity was 126±32,306±58,and 247±37 in the control group,LPS stimulation group,and 17β-estradiol group,respectively.17β-estradiol could decrease the expression of TLR4 mRNA and the transcription activity of NF-кB in LPS-stimulated RAW264.7 cells(P<0.05). Conclusion 17β-estradiol has anti-inflammatory effect via regulation of TLR4 expression and NF-кB transcriptional activity.
- estrogen /
- macrophage /
- NF-кB /
- TLR4

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参考文献(9)

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