莱菔硫烷对膀胱癌细胞增殖抑制作用

李宝龙; 何灿霞; 王凤前; 冯雪; 潘萌; 黄睿; 单毓娟

doi:10.11847/zgggws-2012-28-02-27

莱菔硫烷对膀胱癌细胞增殖抑制作用

doi: 10.11847/zgggws-2012-28-02-27

1.
黑龙江中医药大学药物安全性评价中心, 黑龙江哈尔滨150040;
2.
哈尔滨医科大学;
3.
哈尔滨工业大学

基金项目:

国家自然科学基金(30872106);中国博士后特别资助项目(200902415)

详细信息

作者简介:
李宝龙(1973- ),男,黑龙江青冈人,副教授,博士在读,研究方向:食源性药物的生物活性研究及开发。

通信作者:
单毓娟,E-mail:yujuan72@163.com

中图分类号: R737.14
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- 被引次数: 0
出版历程
- 接收日期: 2010-12-29
- 刊出日期: 2012-02-10

Effects of sulforaphane on proliferation of human bladder cancer cells and its mechanism

1.
Assessment Center for Drug Safety, Heilongjiang University of Traditional Chinese Medicine, Harbin 150040, China

摘要

摘要: 目的研究丝裂原活化蛋白激酶(MAPKs)和磷脂酰肌醇激酶(PI3K)信号途径在莱菔硫烷(sulforaphane,SFN)抑制膀胱癌细胞增殖中的作用。方法噻唑蓝(MTT)法测定膀胱癌细胞的增殖作用,用实时定量PCR方法(re-al time-PCR)测定对环氧化酶(COX-2)mRNA的影响。结果 5～20μmol/L莱菔硫烷能明显抑制膀胱癌细胞的体外增殖、COX-2 mRNA表达;SB202190或LY294002预处理T24细胞,能明显降低莱菔硫烷对T24细胞增殖的抑制作用,提高细胞存活率;SB202190预处理T24细胞1h能完全阻断莱菔硫烷对COX-2 mRNA的抑制作用,而LY294002不影响莱菔硫烷对COX-2 mRNA的抑制作用。结论莱菔硫烷可能是通过活化p38激酶途径和PI3K激酶途径抑制膀胱癌细胞生长,p38激酶信号途径在SFN抑制COX-2 mRNA中起关键作用。
- 莱菔硫烷 /
- 膀胱癌 /
- 丝裂原活化蛋白激酶(MAPK) /
- 环氧化酶(COX-2)
Abstract: Objective To sutdy the effects of mitogen activated protein kinase(MAPK)and phosphatidy linositol 3-kinase(PI3K)on the inhibition of human bladder cancer cell proliferation induced by sulforaphane(SFN). Methods Methyl thiazolyl tetrazolium(MTT)assay was used to detect T24 cell proliferation.The level of cyclooxygenase-2(COX-2) mRNA was determined by quantitative real-time PCR. Results SFN(5-20μmol/L)significantly suppressed the proliferation and the expression of COX-2 mRNA.Pretreatment with inhibitor SB202190 or LY294002,anti-proliferation of SFN on T24 cells was remarkably attenuated by the increase of cell viability.Inhibitor SB202190,but not LY294002,could thoroughly masked the level of COX-2 mRNA downregulated by SFN. Conclusion The results suggest that p38MAPK and PI3K signal pathway are involved in the inhibitory effect of SFN on T24 cells and p38MAPK plays a key role in the inhibition of COX-2 mRNA by SFN.
- sulforaphane(SFN) /
- bladder cancer /
- mitogen activated protein kinase(MAPK) /
- cyclooxygenase-2(COX-2)

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参考文献(8)

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