Effects of bisphenol A on calcium channel currents in dorsal root ganglion neurons of rats
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摘要: 目的 探讨双酚A对大鼠背根神经节(DRG)神经元钙离子通道影响。方法 SD大鼠,周龄4～6周,采用酶消化法急性分离大鼠背根神经节神经元,分别采用全细胞膜片钳技术和激光共聚焦技术记录钙电流和KCl激发钙瞬变的变化。结果 双酚A(0.1、1、10、100μmol/L)呈浓度依赖性抑制大鼠背根神经节神经元钙电流,对钙通道的半数最大抑制浓度(IC50)为11.41μmol/L;10μmol/L双酚A显著改变Ca2+通道的激活特性,电流激活曲线去极化方向移动(P<0.05),该浓度双酚A同样可以抑制50 mmol/L KCL激发瞬时胞内钙浓度增加。结论 双酚A对大鼠背根神经节神经元钙离子通道具有抑制作用。Abstract: Objective To explore the effects of bisphenol A(BPA)on calcium currents in dorsal root ganglion(DRG) neuron cells of rats. Methods Ca2+ currents were obtained in DRG neurons by whole-cell patch clamp technique;calcium transients induced by 50 mmol/L KCl were also observed in the neurons with laser scanning confocal microscopy assay,and the effects of bisphenol A on calcium channels were observed. Results BPA at the concentrations of 0.1,1,10,and 100 μmol/L significantly inhibited calcium current in DRG neurons cells,with an IC 50 of 11.41μmol/L.BPA shifted the activation curve of calcium currents toward a depolarizing direction and increased the slope factor of the curve.Additionally, 10μmol/L BPA inhibited the increment of intracellular Ca2+ concentration([Ca2+]i)induced by 50 mmol/L KCl. Conclusion BPA has significant inhibitory effects on calcium current and calcium transient invoked by KCl in dorsal root ganglion neurons.
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Key words:
- bisphemol A /
- calcium channel /
- patch clamp /
- laser scanning confocal microscopy /
- calcium transient
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