Abstract: Objective To investigate the acceptability of male circumcision(MC)and its influencing factors among men who have sex with men(MSM)in Guiyang municipality to promote the acceptability of male circumcision for the prevention of sexually transmitted diseases. Methods Questionnaire interviews were administered to 357 MSM recruited and the results of 302 MSM without MC experience were analyszed. Results Among the MSM,57.3% (173/302)had the first sex at the average age of 21.7 years and 61.6% (186/302)had the first sex with men.For the MSM the average number of male partners in the latest 6 months was 4.2 and 94.4% (285/302)of MSM had anal sex with men but only 31.4% (89/302)used a condom everytime.There were 17.9% (54/302)of MSM having anal sex with female,of which only 35.1% (19/54)used a condom everytime.Totally 48.01% (145/302)of the MSM accepted MC.Multivariate logistic regression analyses showed the influencing factors included knowing the effect of MC on prevention of sexually transmitted diseases(P=0.016),with self-diagnosed prepuce or phimosis(P=0.006),awareness of effect of MC on quality of sex(P=0.000),and believing in good quality of MC(P=0.000). Conclusion The knowledge about effects of MC on sexual function and prevention of sexually transmitted diseases are related to the acceptability on MC among MSM in Guiyang city.