Abstract: Objective To explore the status and influencing factors of attitude toward lesbian,gay,bisexality,and transgender(LGGT)among college stuldents and to provide implication for sex education in the students. Methods Totlly 1 762 college students fom 5 universities in Chongqing and Chengdu city were selected for the questionnaire survey. Results There were 219 students(16.5%)having homosexual attraction themselves,154(8.5%)reporting having homosexual contact,and 74(4.2%)reporting gender variant identity.Among the students,the percentage of reporting the acceptance was 12.4% (218)for having intercourse with the same sex,12.6% (222)for having romantic love with transgender,16.8% (207)for changing sex,19.1% (347)for having sex drive toward the same gender, 26.2% (461)for homosexuality marriage,28.9% (510)for bisexuality being accordant to social norms,and 35.1% (618)for homosexuality being accordant to social norms. Conclusion Chinese college students have negative and conservative attitude tow ard LGBT,which could be influenced by gender,grade,residence and parents'education background,rather than influenced by sex education experience,discipline and family structure.Besides,gender is an important moderate factor for the impact of parents'education level and residence on the attitude toward LGBT among college students.