Abstract: Objective To investigate hereditary character of cytochrome P450 1B1(CYP1B1)codon 432 among Guangxi Zhuang population. Methods Gene polymorphism of CYP1B1 codon 432 was detected by di-allele-specificamplification with artificially modified primers(diASA-AMP)in 288 healthy Guangxi Zhuang people.The genotype frequencies of CYP1B1 codon 432 in the population were compared with other populations in China,other countries and regions. Results The frequency of allele C in CYP1B1 gene Leu 432 Val was high in both female(89.1%)and male population(91.4%).The prevalence rates of CYP1B1 Leu 432 Val genotypes CC,CG,and GG were 78.3%,21.3%, and 0 in the female population,and those in male population were 83.6%,15.6%,and 0.8%,respectively.There was no difference between female and male population in allele and genotype frequency of CYP1B1 Leu 432 Val in the population.The frequency of genotype distribution in the population had no obvious differences compared with Korea popoulation,but had significant differences compared with other populations in Jiangsu,Shanghai,Sichuan(P<0.05),Japan, India,Poland,Nigeria,and America(P<0.01). Conclusion Gene polymorphism of CYP1B1 in codon 432 has no gender difference in Guangxi Zhuang population,and the distributions of the gene polymorphism in the population are different from those in other regions of China and other countries.