Abstract:
Objective To establish an indirect competitive enzyme-linked immunosorbent assay (ic-ELISA) using monoclonal antibody of bisphenol A (BPA) for the detection of serum BPA.
Methods Totally 176 pupils aged 8 – 10 years were selected from three primary schools in suburban area of Nanchang city of Jiangxi province and serum BPA of the pupils were determined with ic-ELISA established.
Results The detection limit of the established ic-ELISA method was 0.43 ng/mL. The recovery rates of BPA in serum spiked at 4 different levels were between 70.1% and 87.8% with the variation coefficients from 4.79% to 9.41%. BPA was detected in 95 of the 176 serum samples and the detection rate was 54%. The serum BPA content ranged from non-detectable to 26.48 ng/mL for all the samples. The detection rate of serum BPA was higher among the boy pupils than among the girl pupils, but the detection rate was not significantly different among the pupils of various age groups (P = 0.195).
Conclusion The established ic-ELISA could meet the requirement of serum BPA detection among children. BPA was detectable in serum of children in Nanchang city of Jiangxi province and relatively higher serum BPA content was detected in some of the children, suggesting studies are needed to explore the contamination source of BPA.
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