B族维生素片中维生素B<sub>12</sub>电感耦合等离子体质谱法间接测定

王国玲; 邵立君; 聂宏骞; 杨路平; 王锡宁

doi:10.11847/zgggws1114114

B族维生素片中维生素B₁₂电感耦合等离子体质谱法间接测定

Indirect detection of VB₁₂ in vitamin B complex tablets with inductively coupled plasma mass spectrometry

摘要

摘要:
目的建立电感耦合等离子体质谱（ICP-MS）法间接测定B族维生素片中维生素B₁₂含量的方法。
方法样品经微波消解后，采用在线加入内标校正基体效应，用ICP-MS测定钴离子浓度间接测定样品中维生素B₁₂的含量。
结果本方法在1.0～100.0 μg/L线性范围内具有良好的线性关系（r > 0.999），加标回收率为95.0 %～104.0 %，相对标准偏差（RSD）为1.17%～3.03%，方法检出限为0.014 mg/kg，定量下限为0.041 mg/kg。
结论本方法简单、灵敏、准确，适用于B族维生素片中维生素B₁₂的测定。

Abstract:
Objective To establish an inductively coupled plasma mass spectrometry (ICP-MS) method for indirect detection of vitamin B₁₂ content in vitamin B complex tablets.
Methods The sample was pretreated with microwave digestion and the matrix effect was corrected with the in-line addition of internal standards. Vitamin B₁₂ was determined indirectly with the detection of cobalt ions in the sample.
Results The linearity range of the established method was satisfying for the concentration of 1.0 – 100 .0 g/L, with the the correlation coefficients of greater than 0.999. The average recoveries were 95.0% – 104.0%, with a relative standard deviation of 1.17% – 3.03%. The limit of detection (LOD) was 0.014 mg/kg and the limit of quantification (LOQ) was 0.041 mg/kg.
Conclusion The method is simple, sensitive, accurate, and applicable for the detection of vitamin B₁₂ content in vitamin B complex tablets.

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B族维生素片中维生素B12电感耦合等离子体质谱法间接测定

Indirect detection of VB12 in vitamin B complex tablets with inductively coupled plasma mass spectrometry

B族维生素片中维生素B₁₂电感耦合等离子体质谱法间接测定

Indirect detection of VB₁₂ in vitamin B complex tablets with inductively coupled plasma mass spectrometry