Objective  To investigate the association of two single nucleotide polymorphisms (SNPs, rs10205923 and rs16822633) in insulin receptor substrate-1 gene (IRS-1) and one SNP (rs9521509) in insulin receptor substrate-2 gene (IRS-2) with coronary artery disease (CAD) susceptibility among Chinese Han population in Guangdong province.

  Methods  A total of 783 CAD patients and 749 healthy individuals were genotyped using SNPscan^TM multiple SNP genotyping assay; the association between the three SNP loci and CAD was tested with Chi-square test and logistic regression model.

  Results  There were no statistically significant differences in allelic and genotypic frequency distribution of the three polymorphic loci between the patients with CAD and the health controls (all P > 0.05). Multivariate logistic regression analysis did not reveal significant effect of SNPs of the three loci on CAD susceptibility under three genetic models (additive, dominant, and recessive), with the P values ranging from 0.406 to 0.949 after adjusting for several confounding factors (age, hypertension, diabetes, smoking history, and body mass index). Further haplotype analysis on the two SNPs (rs10205923 and rs16822633) in IRS-1 did not find any haplotype that conferred significant effect on CAD susceptibility, with the covariate adjusted P values ranging from 0.439 to 0.941.

  Conclusion  This study suggests that SNPs of IRS-1 rs10205923, rs16822633 and IRS-2 rs9521509 do not associate with the CAD susceptibility among Chinese Han population in Guangdong province.