Objective  To screen and qualitatively identify unknown intracellular interactive proteins of Nelson Bay orthoreovirus μNS protein for providing a reference to the development of anti-reovirus drugs.

  Methods  The plasmid pEFHAM3 and empty plasmids containing the M3 gene fragment were constructed and transfected into 293T cells, respectively. The μNS protein encoded by the M3 gene fragment and its interactive protein were obtained with immunoprecipitation method. The proteins of the two groups were detected qualitatively and differences in protein components were analyzed.

  Results  Totally 9 differential proteins were qualitatively detected, including serine/threonine protein kinase, arginine synthase, keratin 14, mitochondrial Rho GTPase 1, histone H2B, 40S ribosomal protein S4, ribose protein L26, actin-2, and an uncharacterized protein.

  Conclusion  One or more of the nine differential proteins may be involved in the replication of Nelson Bay orthoreovirus inducing human respiratory infection.