D-青霉胺稳定银簇聚集体对大肠杆菌和金黄色葡萄球菌抑制作用

韩毅; 孔令灿; 李耕伟

doi:10.11847/zgggws1133260

D-青霉胺稳定银簇聚集体对大肠杆菌和金黄色葡萄球菌抑制作用

doi: 10.11847/zgggws1133260

1.
无锡市疾病预防控制中心
2.
新乡市粮油饲料产品质量监督检验所

详细信息

作者简介:
韩毅（1982 – ），男，江苏无锡人，高级实验师，硕士，主要从事病原微生物检测和研究工作

中图分类号: R 446.5
计量
- 文章访问数: 225
- HTML全文浏览量: 80
- PDF下载量: 13
- 被引次数: 0
出版历程
- 收稿日期: 2020-11-24
- 网络出版日期: 2021-11-16
- 刊出日期: 2022-02-01

Inhibitory effect of D-penicillamine stabilized silver cluster on Escherichia coli and Staphylococcus aureus

1.
Wuxi Municipal Center for Disease Control and Prevention, Wuxi, Jiangsu Province 214023, China

摘要

摘要: 目的探讨D – 青霉胺稳定的银簇聚集体对大肠杆菌和金黄色葡萄球菌的抑制作用。方法利用红外光谱、X – 射线光电子能谱和俄歇电子谱分析银簇聚集体的结构；并将银簇聚集体与细菌混合培养，探讨银簇聚集体的抑菌作用。利用扫描电子显微镜观察细菌培养后状态。结果银簇聚集体对大肠杆菌最低抑菌浓度（MIC）为1 μg/mL，半抑制浓度IC₅₀为0.86 μg/mL；银簇聚集体对金黄色葡萄球菌无抑制作用，即使银簇聚集体浓度达到大肠杆菌MIC的200倍也无抑菌效果。扫描电子显微镜结果表明，与银簇聚集体培养后，大肠杆菌的细胞膜发生明显破裂，导致细菌死亡。结论银簇聚集体对大肠杆菌具有明显抑制作用，对金黄色葡萄球菌无抑制作用。
- 大肠杆菌 /
- 银簇聚集体 /
- 选择性抑菌
Abstract: Objective To observe inhibitory effect of silver cluster assemblies capped by D-penicillamine on Escherichia coli (E.coli) and Staphylococcus aureus (S.aureus). Methods The structure of D-penicillamine stabilized silver cluster assemblies was examined with infrared spectroscopy, X-ray photoelectron spectroscopy and Auger electron spectroscopy. Then, the E.coli and S.aureus strains were cultivated with various doses of D-penicillamine stabilized silver cluster assemblies. Scanning electron microscope was used to observe the proliferation of the cultivated bacteria. Results The minimum inhibitory concentration (MIC) and the half maximal inhibitory concentration of silver cluster assemblies for E. coli were 1 μg/mL and 0.86 μg/mL; no inhibition effect of silver cluster assemblies on S.aureus at the concentration of 200 times higher than the MIC for E. coli. Observations with scanning electron microscope revealed obvious rupture of the cell membranes of E. coli cultivated with silver cluster assemblies, which may be contributed to the bacterial death. Conclusion Silver cluster assemblies has obvious inhibition effect on E. coli but no such effect on S.aureus.
- Escherichia coli /
- silver cluster assemblies /
- selective bacteriostasis

HTML全文

图 1 D – 青霉胺和银簇聚集体的红外光谱图

下载: 全尺寸图片幻灯片

图 2 银簇聚集体X – 射线光电子能谱（C 1s、N 1s、O 1s、S 2p、Ag 3d）及银簇聚集体俄歇电子谱（Ag MNN）

下载: 全尺寸图片幻灯片

图 3 银簇聚集体对大肠杆菌的抑制曲线

下载: 全尺寸图片幻灯片

图 4 加入银簇聚集体前后大肠杆菌扫描电子显微镜下变化

下载: 全尺寸图片幻灯片

图 5 银簇聚集体扫描电镜图

下载: 全尺寸图片幻灯片

表 1 不同浓度银簇聚集体对大肠杆菌抑制作用（吸光度）

银簇聚集体浓度（μg/mL）	培养时间（h）								菌落计数（CFU）
银簇聚集体浓度（μg/mL）	0	1	2	3	4	5	6	7	菌落计数（CFU）
0.000	0.029	0.038	0.057	0.118	0.175	0.222	0.247	0.274	118
0.081	0.032	0.040	0.053	0.107	0.170	0.208	0.240	0.259
0.161	0.032	0.041	0.052	0.108	0.168	0.229	0.240	0.259	114
0.250	0.031	0.040	0.044	0.088	0.141	0.198	0.241	0.262
0.323	0.034	0.041	0.047	0.083	0.135	0.190	0.233	0.255
0.405	0.032	0.037	0.045	0.069	0.111	0.169	0.229	0.254	100
0.500	0.035	0.039	0.049	0.066	0.096	0.151	0.214	0.248
0.750	0.035	0.037	0.047	0.060	0.067	0.079	0.139	0.196	85
1.008	0.039	0.036	0.047	0.058	0.057	0.052	0.054	0.071	6

下载: 导出CSV

表 2 不同浓度银簇聚集体对金黄色葡萄球菌的抑制作用（吸光度）

银簇聚集体浓度（μg/mL）	培养时间（h）
银簇聚集体浓度（μg/mL）	0	1	2	3	4	5	6	7	24
0	0.037	0.047	0.107	0.181	0.213	0.232	0.242	0.252	0.227
3	0.049	0.050	0.116	0.182	0.202	0.219	0.237	0.252	0.262
6	0.059	0.054	0.121	0.192	0.212	0.225	0.243	0.254	0.258
9	0.080	0.055	0.120	0.186	0.206	0.223	0.236	0.248	0.256
12	0.092	0.054	0.115	0.182	0.202	0.216	0.232	0.242	0.246
15	0.104	0.054	0.116	0.183	0.201	0.219	0.234	0.245	0.241
18	0.139	0.053	0.115	0.188	0.206	0.217	0.233	0.244	0.245
21	0.151	0.046	0.105	0.180	0.195	0.206	0.221	0.230	0.221
24	0.170	0.048	0.110	0.185	0.198	0.204	0.220	0.230	0.217
27	0.173	0.050	0.115	0.188	0.201	0.207	0.223	0.235	0.221
30	0.175	0.058	0.137	0.194	0.208	0.221	0.238	0.251	0.243

下载: 导出CSV

参考文献(8)

[1]	Chen NN, Zheng Y, Yin JJ, et al. Inhibitory effects of silver nanoparticles against adenovirus type 3 in vitro[J]. Journal of Virological Methods, 2013, 193(2): 470 – 477. doi: 10.1016/j.jviromet.2013.07.020
[2]	Trefry JC, Wooley DP. Silver nanoparticles inhibit Vaccinia virus infection by preventing viral entry through a macropinocytosis-dependent mechanism[J]. Journal of Biomedical Nanotechnology, 2013, 9(9): 1624 – 1635. doi: 10.1166/jbn.2013.1659
[3]	Elechiguerra JL, Burt JL, Morones JR, et al. Interaction of silver nanoparticles with HIV-1[J]. Journal of Nanobiotechnology, 2005, 3: 6. doi: 10.1186/1477-3155-3-6
[4]	Xiang DX, Zheng Y, Duan W, et al. Inhibition of A/Human/Hubei/3/2005 (H3N2) influenza virus infection by silver nanoparticles in vitro and in vivo[J]. International Journal of Nanomedicine, 2013, 8: 4103 – 4114.
[5]	Zheng KY, Li KR, Chang TH, et al. Synergistic antimicrobial capability of magnetically oriented graphene oxide conjugated with gold nanoclusters[J]. Advanced Functional Materials, 2019, 29(46): 1904603. doi: 10.1002/adfm.201904603
[6]	Shao W, Lin XF, Min HH, et al. Preparation, characterization, and antibacterial activity of silver nanoparticle-decorated graphene oxide nanocomposite[J]. ACS Applied Materials and Interfaces, 2015, 7(12): 6966 – 6973. doi: 10.1021/acsami.5b00937
[7]	Rajeshkumar S, Malarkodi C. In vitro antibacterial activity and mechanism of silver nanoparticles against foodborne pathogens[J]. Bioinorganic Chemistry and Applications, 2014, 2014: 581890.
[8]	Li WR, Xie XB, Shi QS, et al. Antibacterial activity and mechanism of silver nanoparticles on Escherichia coli[J]. Applied Microbiology and Biotechnology, 2010, 85(4): 1115 – 1112. doi: 10.1007/s00253-009-2159-5