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液相芯片技术在感染性腹泻病原体检测中应用

曹军 管红霞

曹军, 管红霞. 液相芯片技术在感染性腹泻病原体检测中应用[J]. 中国公共卫生, 2022, 38(7): 939-943. doi: 10.11847/zgggws1133735
引用本文: 曹军, 管红霞. 液相芯片技术在感染性腹泻病原体检测中应用[J]. 中国公共卫生, 2022, 38(7): 939-943. doi: 10.11847/zgggws1133735
CAO Jun, GUAN Hong-xia. Application of liquid-phase chip technology in infectious diarrhea pathogen detection[J]. Chinese Journal of Public Health, 2022, 38(7): 939-943. doi: 10.11847/zgggws1133735
Citation: CAO Jun, GUAN Hong-xia. Application of liquid-phase chip technology in infectious diarrhea pathogen detection[J]. Chinese Journal of Public Health, 2022, 38(7): 939-943. doi: 10.11847/zgggws1133735

液相芯片技术在感染性腹泻病原体检测中应用

doi: 10.11847/zgggws1133735
基金项目: 无锡市卫健委项目(T202017);无锡市科技局项目(锡科社[2020]259号 – 24)
详细信息
    作者简介:

    曹军(1976 – ),男,江苏无锡人,副主任技师,本科,研究方向:传染病病原监测

    通讯作者:

    管红霞,E-mail:ghx-331@163.com

  • 中图分类号: R 115

Application of liquid-phase chip technology in infectious diarrhea pathogen detection

  • 摘要:   目的  评价液相芯片技术检测感染性腹泻病原体的应用价值。  方法  于2015年4月 — 2016年4月收集江苏省无锡市人民医院(含儿童医院)肠道门诊就诊的179份腹泻病例粪便标本,采用美国Luminex公司胃肠道病原体检测试剂盒(xTAG GPP)进行检测,并与real-time PCR检测结果进行比较。  结果  xTAG GPP检出9种病原体82例,阳性检出率为45.81 %(82/179),real-time PCR检出9种病原体81例,阳性检出率为45.25 %(81/179),两种检测方法总符合率92.17 %(165/179)。xTAG GPP检测腹泻病原体灵敏度为84.44 %,特异度为93.33 %,阳性预测值为92.68 %,阴性预测值为85.71 %。  结论  与传统的检测方法相比,液相芯片技术检测通量高、速度快,对常见腹泻病原体具有较高的敏感性和特异性,可用于腹泻病的快速诊断。
  • 表  1  xTAG GPP和real-time PCR检测病原及目标基因

    xTAG GPP目标基因
    病原中文名称病原英文名称real-time PCRTPT real-time PCR
    腺病毒40/41型 Adenovirus 40/41 hexon(内部使用) hexon [3-4]
    诺如病毒GI/GII Norovirus GI/GII ORF1-ORF2 junction(内部使用) RDRP of GI [5]; RDRP and ORF1-ORF2 junction of GII a[6-7]
    A组轮状病毒 Rotavirus A NSP3(内部使用) VP6 [7]
    弯曲菌 Campylobacter C. jejuni hipO; C. coli glyA(内部使用) glyA of C. jejuni and C. coli [8],cadF [9]
    艰难梭菌(毒素A/B型) C. difficile toxin A/B tcdA,tcdB(内部使用) tcdA,tcdB [10-12]
    肠出血性大肠埃希菌O157 E. coli O157
    肠产毒性大肠埃希菌LT/ST型 ETEC LT/ST elt,estA(STh),estB(STp)(内部使用) elt,estA(STh),estB(STp)[13]
    产志贺毒素大肠埃希菌stx1/stx2 STEC stx1/stx2 stx1,stx2(内部使用) stx1,stx2 [13]
    肠侵袭性大肠埃希菌/志贺菌 EIEC/Shigella ipaH(内部使用) virA [14]
    沙门菌 Salmonella ttrRSBCA(内部使用),培养法 ompC [15]
    霍乱弧菌 V. cholerae ompW(内部使用) epsM [16]
    小肠结肠炎耶尔森氏菌 Y. enterocolitica ystA(内部使用) ail [17]
    隐孢子虫 Cryptosporidium 18S rRNA gene(内部使用) 18S rRNA gene [18-19]
    痢疾阿米巴 E. histolytica SSU rRNA gene(内部使用) SSU rRNA gene [20-21]
    蓝氏贾第鞭毛虫 G. lamblia SSU RNA gene(内部使用) hsp [18]
      注:a 使用两对引物对诺如病毒GII进行不一致分析,任何一对引物为阳性,则判定标本为阳性。
    下载: 导出CSV

    表  2  不同方法病原体的检测结果

    病原体培养法xTAG GPPreal-time PCR总阳性数 a总阳性率(%)
    阳性数阳性数阳性率(%)阳性数阳性率(%)
    诺如病毒GI/GII型(Norovirus GI/GII) 25 13.97 25 13.97 25 13.97
    A组轮状病毒(Rotavirus A) 23 12.85 23 12.85 24 12.85
    腺病毒40/41型(Adenovirus 40/41) 3 1.68 4 2.23 4 2.23
    沙门菌(Salmonella 17 5 2.79 8 4.47 17 9.50
    弯曲菌(Campylobacter 9 5.03 10 5.59 10 5.59
    肠产毒性大肠埃希菌(ETEC) 2 1.12 2 1.12 2 1.12
    艰难梭菌(毒素A/B)(C. difficile toxin A/B 4 2.23 4 2.23 4 2.23
    志贺菌(Shigella 8 4.47 6 3.35 6 3.35
    霍乱弧菌(V. cholerae 2 1.12 2 1.12 2 1.12
    合计 b 17 82 45.81 81 45.25 90 50.28
      注:a 阳性数为培养法、xTAG GPP和real-time PCR检测为阳性标本的总和;b 因部分标本同时感染两种或多种病原体,不计算此类靶标在标本中的阳性检出率。
    下载: 导出CSV

    表  3  xTAG GPP和real-time PCR检测不同病原体检测的灵敏度和特异度

    病原体金标准axTAG GPP灵敏度(%)特异度(%)阳性预测值(%)阴性预测值(%)real-time PCR灵敏度(%)特异度(%)阳性预测值(%)阴性预测值(%)
    PNPN
    Norovirus GI P 6 0 100.00 100.00 100.00 100.00 6 0 100.00 100.00 100.00 100.00
    N 0 173 0 173
    Norovirus GII P 20 0 100.00 99.37 95.24 100.00 21 0 100.00 100.00 100.00 100.00
    N 1 158 0 158
    Rotavirus A P 23 0 100.00 100.00 100.00 100.00 23 0 100.00 100.00 100.00 100.00
    N 0 156 0 156
    Adenovirus 40/41 P 3 1 75.00 100.00 100.00 99.43 4 0 100.00 100.00 100.00 100.00
    N 0 175 0 175
    Salmonella P 5 12 29.41 100.00 100.00 93.10 8 9 47.06 100.00 100.00 94.74
    N 0 162 0 162
    Campylobacter P 9 1 90.00 100.00 100.00 99.41 10 0 100.00 100.00 100.00 100.00
    N 0 169 0 169
    ETEC(LT) P 1 0 100.00 100.00 100.00 100.00 1 0 100.00 100.00 100.00 100.00
    N 0 178 0 178
    ETEC(ST) P 1 0 100.00 100.00 100.00 100.00 1 0 100.00 100.00 100.00 100.00
    N 0 178 0 178
    C. difficile toxin A/B P 4 0 100.00 100.00 100.00 100.00 4 0 100.00 100.00 100.00 100.00
    N 0 175 0 175
    Shigella P 6 0 100.00 98.84 75.00 100.00 6 0 100.00 100.00 100.00 100.00
    N 2 171 0 173
    V. cholerae P 2 0 100.00 100.00 100.00 100.00 2 0 100.00 100.00 100.00 100.00
    N 0 177 0 177
    合计 P 76 14 84.44 93.33 92.68 85.71 81 9 90.00 100.00 100.00 90.82
    N 6 84 0 89
      注:a 金标准为按照本研究方法中阳性的判定原则得到的最终结果;P为阳性;N为阴性。
    下载: 导出CSV
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  • 收稿日期:  2020-12-29
  • 网络出版日期:  2022-03-23
  • 刊出日期:  2022-07-10

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