孕妇外周血及脐带血红细胞甲基汞含量孕期内变化情况检测分析

陈星; 罗佳瑶; 陈欢; 陈曦; 李媛媛

doi:10.11847/zgggws1136158

孕妇外周血及脐带血红细胞甲基汞含量孕期内变化情况检测分析

doi: 10.11847/zgggws1136158

陈星^{1, （},
罗佳瑶¹,
陈欢¹,
陈曦²,
李媛媛^1, ,

1.
华中科技大学同济医学院公共卫生学院环境与健康教育部重点实验室，湖北武汉 430030
2.
中国疾病预防控制中心环境与健康相关产品安全所

基金项目: 国家自然科学基金（42077398）

详细信息

作者简介:
陈星（1999 – ），男，贵州安顺人，本科在读，研究方向：环境与健康方面

通讯作者:
李媛媛，E-mail：liyunayuan@hust.edu.cn

（罗佳瑶为本文并列第一作者）
中图分类号: R 12
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- 被引次数: 0
出版历程
- 接收日期: 2021-07-12
- 网络出版日期: 2022-07-22
- 刊出日期: 2022-08-31

Changes in erythrocyte methylmercury among women during pregnancy: a monocentric survey and comparison study

CHEN Xing^{1, （},
LUO Jia-yao¹,
CHEN Huan¹,
CHEN Xi²,

1.
School of Public Health, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, Hubei Province, 430030 China

摘要

摘要: 目的调查普通孕妇不同孕期外周血及新生儿脐带血红细胞中甲基汞浓度变化及相关性。方法于2019年1 — 4月在湖北省武汉市招募79名孕妇，采集孕妇孕早期[孕（12 ± 2）周]和孕晚期[孕（37 ± 2）周]外周血及新生儿脐带血，分离红细胞，采用高效液相色谱 – 电感耦合等离子体质谱联用方法检测红细胞内甲基汞浓度。结果本研究孕妇孕早、晚期及脐带血红细胞中甲基汞浓度的几何均数分别为0.322、0.238、0.515 μg/g，四分位数间距分别为0.188～0.728、0.120～0.549、0.322～1.099 μg/g，孕早期甲基汞含量显著高于孕晚期（P < 0.05），孕晚期甲基汞含量显著低于脐带血（P < 0.05），孕早期甲基汞含量也显著低于脐带血（P < 0.05），孕早、晚期血及脐带血甲基汞含量之间两两呈现强正相关性（r = 0.763、0.866、0.854）。结论孕妇孕早期、孕晚期血和新生儿脐带血红细胞中甲基汞含量存在明显相关性；脐带血红细胞甲基汞含量显著高于孕妇外周血，提示可能存在富集现象。
- 孕期汞暴露 /
- 甲基汞 /
- 红细胞汞
Abstract: Objective To investigate changes in peripheral erythrocyte methylmercury among pregnant women during different gestational periods and the methylmercury in neonatal umbilical cord blood erythrocyte. Methods Totally 79 pregnant women having prenatal examination were recruited at a women′s and children′s health care center in Wuhan city during January – April, 2019. The women′s peripheral blood samples were collected at the first trimester (12 ± 2 weeks of gestation) and the third trimester (37 ± 2 weeks of gestation) and neonatal umbilical cord blood samples were also collected. Red blood cells of the samples were separated and concentrations of methylmercury in the cells were detected with high performance liquid chromatography-inductively coupled plasma mass spectrometry (HPLC-ICP-MS). Results The geometric means of erythrocyte methylmercury of all the women were 0.322 μg/g (interquartile range [IRQ]: 0.188 – 0.728 μg/g) for the samples of first trimester, 0.238 μg/g (IRQ: 0.120 – 0.549 μg/g ) for those of third trimester, and 0.515 μg/g (IRQ: 0.322 – 1.099 μg/g) for neonatal umbilical cord samples. The erythrocyte methylmercury of the first trimester samples was significantly higher than that of the third trimester samples (P < 0.05); while, the erythrocyte methylmercury of both the first trimester and the third trimester samples were significantly lower than that of neonatal umbilical cord samples (both P < 0.05). The erythrocyte methylmercury of the umbilical cord blood samples, the first trimester samples and the third trimester samples were closely correlated with each other, with the correlation coefficients of 0.763, 0.866, and 0.854, respectively. Conclusion The erythrocyte methylmercury of pregnant women during first and third trimester and that of neonatal umbilical cord blood are significantly correlated with each other. Higher erythrocyte methylmercury of neonatal umbilical cord blood than that of women during pregnancy suggests a possible enrichment of methyl mercury.
- mercury exposure during pregnancy /
- methyl mercury /
- erythrocyte mercury
注释:

1) （罗佳瑶为本文并列第一作者）

HTML全文

表 1 孕妇不同孕期外周血和新生儿脐带血红细胞汞含量分布（μg/g）

时期	G	P₂₅～P₇₅	M	范围
孕早期	0.322	0.188～0.728 ^a	0.421	0.014～1.652
孕晚期	0.238	0.120～0.549 ^b	0.341	0.006～1.890
脐带血	0.515	0.322～1.099 ^c	0.704	0.018～2.691
注：与孕晚期比较，a P < 0.05；与脐带血比较，b P < 0.05；与孕早期比较，c P < 0.05。

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表 2 孕妇不同孕期外周血和新生儿脐带血红细胞汞含量相关系数

样本	孕早期	孕晚期	脐带血
孕早期	1.000	0.763 ^a	0.854 ^a
孕晚期		1.000	0.866 ^a
脐带血			1.000
注：a P < 0.05。

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表 3 各地区脐带血与母血汞含量比值比较

汞含量	地区	脐带血（μg/L）	母血（μg/L）	比值
总汞	Arctic Canada^[7]	5.80（2.7）	2.96（1.66）	1.49（1.40）
	Bochum Germany^[8]	1.48	0.44	3.36
	South African^[9]	1.20	0.65	1.85
	Eastern Slovakia^[10]	0.53	0.50	1.24
	松原市^[11]	2.87（2.66）	1.58（1.34）	2.28
	山东省^[12]	1.21	0.68	1.73
	韩国^[13]	5.87	3.99	1.47
甲基汞	Arctic Canada^[7]	4.90（2.5）	2.20（1.3）	1.86（1.64）
	Eastern Slovakia^[10]	0.32	0.22	1.80
	武汉[本研究孕早期] ^a	0.75（0.52）	0.48（0.32）	1.96（1.56）
	武汉[本研究孕晚期] ^a	0.75（0.52）	0.40（0.24）	2.90（1.89）
注：括号外为算数均数，括号内为几何均数；a 本研究为红细胞甲基汞（μg/g）。

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