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紫外线A联合异硫氰酸盐杀菌效果评价

侯衍飞 韩惠芳 高桥章 刘根义

侯衍飞, 韩惠芳, 高桥章, 刘根义. 紫外线A联合异硫氰酸盐杀菌效果评价[J]. 中国公共卫生, 2022, 38(7): 884-886. doi: 10.11847/zgggws1136417
引用本文: 侯衍飞, 韩惠芳, 高桥章, 刘根义. 紫外线A联合异硫氰酸盐杀菌效果评价[J]. 中国公共卫生, 2022, 38(7): 884-886. doi: 10.11847/zgggws1136417
HOU Yan-fei, HAN Hui-fang, Takahashi Akira, . Bactericidal effect of UVA combined with isothiocyanate on enteropatho-genic Escherichia coli and Vibrio parahaemolyticus[J]. Chinese Journal of Public Health, 2022, 38(7): 884-886. doi: 10.11847/zgggws1136417
Citation: HOU Yan-fei, HAN Hui-fang, Takahashi Akira, . Bactericidal effect of UVA combined with isothiocyanate on enteropatho-genic Escherichia coli and Vibrio parahaemolyticus[J]. Chinese Journal of Public Health, 2022, 38(7): 884-886. doi: 10.11847/zgggws1136417

紫外线A联合异硫氰酸盐杀菌效果评价

doi: 10.11847/zgggws1136417
基金项目: 济宁医学院2016年博士启动基金(600509001);2021年大学生创新训练计划项目(cx2021149)
详细信息
    作者简介:

    侯衍飞(1985 – ),男,山东济宁人,讲师,博士,研究方向:营养与环境卫生学

    通讯作者:

    刘根义,E-mail:genyiliu@163.com

  • 中图分类号:  R 117;R 155.3

Bactericidal effect of UVA combined with isothiocyanate on enteropatho-genic Escherichia coli and Vibrio parahaemolyticus

  • 摘要:   目的  研究紫外线A发光二极管(UVA-LED)与植物化学物烯丙基异硫氰酸盐(AITC)联合对两种致腹泻病原菌的杀菌效果。  方法  以肠致病性大肠杆菌和副溶血性弧菌两种致腹泻病原菌为受试对象,采用不同浓度的烯丙基异硫氰酸盐与UVA365 nm-LED结合进行二因素3 × 2析因设计杀菌实验,以菌落形成单位(CFU)计数细菌存活量并计算各自存活率对数值,然后进行析因设计的方差分析,并做交互效应图。  结果  单独AITC的杀菌效果随着浓度的增高而逐渐增强(P < 0.05),而且UVA365 nm -LED与AITC联合对2种致病菌的效应差异都具有统计学意义(P < 0.05)。UVA-LED和1 mg/mL AITC联用时副溶血性弧菌的存活率降低至 – 6.15 lg。  结论  UVA-LED与烯丙基异硫氰酸盐的联用,对肠致病性大肠杆菌和副溶血弧菌的杀菌效果都存在显著的协同作用。
  • 图  1  UVA与不同浓度AITC联合对大肠杆菌交互效应

    注:与80 µg/mL组比较,a P < 0.05;与无UVA组比较,b P < 0.05,n = 5。

    图  2  UVA与AITC联合对副溶血性弧菌交互效应

    注:与40 µg/mL组比较,a P < 0.05;与无UVA组比较,b P < 0.05,n = 5。

    表  1  UVA与AITC联用对大肠杆菌存活率的一般线性模型方差分析表

    项目Ⅲ类平方和自由度均方F P
    修正模型 42.532 a 5 8.506 648.654 0.000
    截距 61.535 1 61.535 4692.402 0.000
    UVA照射 18.963 1 18.963 1446.069 0.000
    AITC 17.281 2 8.640 658.868 0.000
    UVA照射x AITC 6.288 2 3.144 239.731 0.000
    误差 0.315 24 0.013
    总计 104.381 30
    修正后总计 42.846 29
      注:a R2 = 0.993,调整后R2 = 0.991。
    下载: 导出CSV

    表  2  UVA与AITC联用对副溶血弧菌存活率的一般线性模型方差分析表

    项目Ⅲ类平方和自由度均方F P
    修正模型 118.983 a 5 23.797 96.985 0.000
    截距 130.106 1 130.106 530.262 0.000
    UVA照射 56.317 2 28.159 114.764 0.000
    AITC 51.954 1 51.954 211.745 0.000
    UVA照射x AITC 10.711 2 5.356 21.827 0.000
    误差 5.889 24 0.245
    总计 254.978 30
    修正后总计 124.871 29
      注:a R2 = 0.973,调整后R2 = 0.961。
    下载: 导出CSV
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出版历程
  • 收稿日期:  2021-08-03
  • 网络出版日期:  2021-12-30
  • 刊出日期:  2022-07-10

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