紫外线A联合异硫氰酸盐杀菌效果评价

侯衍飞; 韩惠芳; 高桥章; 刘根义

doi:10.11847/zgggws1136417

紫外线A联合异硫氰酸盐杀菌效果评价

doi: 10.11847/zgggws1136417

1.
济宁医学院公共卫生学院营养与食品卫生学教研室, 山东济宁 272067
2.
日本德岛大学营养生命科学教育部预防环境营养学分野

基金项目: 济宁医学院2016年博士启动基金（600509001）；2021年大学生创新训练计划项目(cx2021149)

详细信息

作者简介:
侯衍飞（1985 – ），男，山东济宁人，讲师，博士，研究方向：营养与环境卫生学

通讯作者:
刘根义，E-mail：genyiliu@163.com

中图分类号: R 117；R 155.3
计量
- 文章访问数: 578
- HTML全文浏览量: 138
- PDF下载量: 90
- 被引次数: 0
出版历程
- 接收日期: 2021-08-03
- 网络出版日期: 2021-12-30
- 刊出日期: 2022-07-01

Bactericidal effect of UVA combined with isothiocyanate on enteropatho-genic Escherichia coli and Vibrio parahaemolyticus

1.
Department of Nutrition and Food Hygiene, School of Public Health, Jining Medical University, Jining, Shandong Province 272067, China

摘要

摘要: 目的研究紫外线A发光二极管（UVA-LED）与植物化学物烯丙基异硫氰酸盐（AITC）联合对两种致腹泻病原菌的杀菌效果。方法以肠致病性大肠杆菌和副溶血性弧菌两种致腹泻病原菌为受试对象，采用不同浓度的烯丙基异硫氰酸盐与UVA_{365 nm}-LED结合进行二因素3 × 2析因设计杀菌实验，以菌落形成单位（CFU）计数细菌存活量并计算各自存活率对数值，然后进行析因设计的方差分析，并做交互效应图。结果单独AITC的杀菌效果随着浓度的增高而逐渐增强（P < 0.05），而且UVA_{365 nm} -LED与AITC联合对2种致病菌的效应差异都具有统计学意义（P < 0.05）。UVA-LED和1 mg/mL AITC联用时副溶血性弧菌的存活率降低至 – 6.15 lg。结论 UVA-LED与烯丙基异硫氰酸盐的联用，对肠致病性大肠杆菌和副溶血弧菌的杀菌效果都存在显著的协同作用。
- 紫外线A发光二极管 /
- 烯丙基异硫氰酸盐 /
- 协同作用 /
- 杀菌
Abstract: Objective To study bactericidal effect of ultraviolet A – light emitting diode (UVA-LED) irradiation combined with phytochemical of allyl isothiocyanate (AITC) on two diarrhea-causing pathogens. Methods Two diarrhea-causing pathogenic bacteria, enteropathogenic Escherichia coli (ETEC) and Vibrio parahaemolyticus (VP), were used as the subjects. Bactericidal experiments in 3 × 2 factorial design were performed with the combined treatment of different concentrations of AITC and UVA_{365 nm}-LED irradiation; and the bacteria survival was assessed with colony form unit (CFU) and logarithm value of survival rate was calculated. The interactive effect of AITC and UVA_{365 nm}-LED irradiation was evaluated with analysis of variance for factorial design and illustrated with schematic diagram. Results The bactericidal effect of AITC alone was gradually increased with the increment of concentration (P < 0.05). The effect of the combination of UVA-LED irradiation with AITC on the two pathogenic bacteria were statistically significant (P < 0.05). When UVA_{365 nm} irradiation was combined with 1 mg/ml AITC, the survive ratio of Vibrio parahaemolyticus was even reduced to – 6.15 lg. Conclusion Synergistic bactericidal effect exists for combined treatment of UVA-LED irradiation and AITC on ETEC and VP.
- ultraviolet A – light emitting diode /
- allyl isothiocyanate /
- synergistic effect /
- bactericidal

HTML全文

图 1 UVA与不同浓度AITC联合对大肠杆菌交互效应

注：与80 µg/mL组比较，a P < 0.05；与无UVA组比较，b P < 0.05，n = 5。

下载: 全尺寸图片幻灯片

图 2 UVA与AITC联合对副溶血性弧菌交互效应

注：与40 µg/mL组比较，a P < 0.05；与无UVA组比较，b P < 0.05，n = 5。

下载: 全尺寸图片幻灯片

表 1 UVA与AITC联用对大肠杆菌存活率的一般线性模型方差分析表

项目	Ⅲ类平方和	自由度	均方	F 值	P 值
修正模型	42.532 ^a	5	8.506	648.654	0.000
截距	61.535	1	61.535	4692.402	0.000
UVA照射	18.963	1	18.963	1446.069	0.000
AITC	17.281	2	8.640	658.868	0.000
UVA照射x AITC	6.288	2	3.144	239.731	0.000
误差	0.315	24	0.013
总计	104.381	30
修正后总计	42.846	29
注：a R² = 0.993，调整后R² = 0.991。

下载: 导出CSV

表 2 UVA与AITC联用对副溶血弧菌存活率的一般线性模型方差分析表

项目	Ⅲ类平方和	自由度	均方	F 值	P 值
修正模型	118.983 ^a	5	23.797	96.985	0.000
截距	130.106	1	130.106	530.262	0.000
UVA照射	56.317	2	28.159	114.764	0.000
AITC	51.954	1	51.954	211.745	0.000
UVA照射x AITC	10.711	2	5.356	21.827	0.000
误差	5.889	24	0.245
总计	254.978	30
修正后总计	124.871	29
注：a R² = 0.973，调整后R² = 0.961。

下载: 导出CSV

参考文献(13)

[1]	Li J, Hirota K, Yumoto H, et al. Enhanced germicidal effects of pulsed UV - LED irradiation on biofilms[J]. Journal of Applied Microbiology, 2010, 109(6): 2183 – 2190. doi: 10.1111/j.1365-2672.2010.04850.x
[2]	Rezaie A, Leite GGS, Melmed GY, et al. Ultraviolet A light effectively reduces bacteria and viruses including coronavirus[J]. PLoS One, 2020, 15(7): e0236199. doi: 10.1371/journal.pone.0236199
[3]	Qi WH, Zhu SM, Shitu A, et al. Low concentration peroxymonosulfate and UVA - LED combination for E. coli inactivation and wastewater disinfection from recirculating aquaculture systems[J]. Journal of Water Process Engineering, 2020, 36: 101362. doi: 10.1016/j.jwpe.2020.101362
[4]	Shirai A, Aihara M, Takahashi A, et al. Synergistic antimicrobial activity based on the combined use of a gemini - quaternary ammonium compound and ultraviolet-A light[J]. Journal of Photochemistry and Photobiology B: Biology, 2014, 130: 226 – 233. doi: 10.1016/j.jphotobiol.2013.11.027
[5]	Bahmid NA, Heising J, Fogliano V, et al. Packaging design using mustard seeds as a natural antimicrobial: a study on inhibition of Pseudomonas fragi in liquid medium[J]. Foods, 2020, 9(6): 789. doi: 10.3390/foods9060789
[6]	Lu ZJ, Dockery CR, Crosby M, et al. Antibacterial activities of wasabi against Escherichia coli O157: H7 and Staphylococcus aureus[J]. Frontiers in Microbiology, 2016, 7: 1403.
[7]	Kramer B, Wunderlich J, Muranyi P. Impact of volatile allyl isothiocyanate on fresh produce[J]. Food Packaging and Shelf Life, 2018, 16: 220 – 224. doi: 10.1016/j.fpsl.2018.04.004
[8]	Li YB, Liu YX, Zhang ZP, et al. Allyl isothiocyanate (AITC) triggered toxicity and FsYvc1 (a STRPC family member) responded sense in Fusarium solani[J]. Frontiers in Microbiology, 2020, 11: 870. doi: 10.3389/fmicb.2020.00870
[9]	Romeo L, Iori R, Rollin P, et al. Isothiocyanates: an overview of their antimicrobial activity against human infections[J]. Molecules, 2018, 23(3): 624. doi: 10.3390/molecules23030624
[10]	Lin CM, Preston III JF, Wei CI. Antibacterial mechanism of allyl isothiocyanate[J]. Journal of Food Protection, 2000, 63(6): 727 – 734. doi: 10.4315/0362-028X-63.6.727
[11]	Kvam E, Benner K. Mechanistic insights into UV - A mediated bacterial disinfection via endogenous photosensitizers[J]. Journal of Photochemistry and Photobiology B:Biology, 2020, 209: 111899. doi: 10.1016/j.jphotobiol.2020.111899
[12]	Feyissa Q, Xu F, Ibrahim Z, et al. Synergistic bactericidal effects of pairs of photosensitizer molecules activated by ultraviolet A light against bacteria in plasma[J]. Transfusion, 2021, 61(2): 594 – 602. doi: 10.1111/trf.16180
[13]	Park MY, Kang DH. Antibacterial activity of Caffeic acid combined with UV - A light against Escherichia coli O157: H7, Salmonella enterica serovar typhimurium, and Listeria monocytogenes[J]. Applied and Environmental Microbiology, 2021, 87(15): e00631 – 21.