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氯乙烯染毒、高脂饮食及联合作用对小鼠肠道菌群影响

郝岩 陈诗奇 赵晓田 沈永梅 仇玉兰

郝岩, 陈诗奇, 赵晓田, 沈永梅, 仇玉兰. 氯乙烯染毒、高脂饮食及联合作用对小鼠肠道菌群影响[J]. 中国公共卫生, 2022, 38(11): 1455-1459. doi: 10.11847/zgggws1136689
引用本文: 郝岩, 陈诗奇, 赵晓田, 沈永梅, 仇玉兰. 氯乙烯染毒、高脂饮食及联合作用对小鼠肠道菌群影响[J]. 中国公共卫生, 2022, 38(11): 1455-1459. doi: 10.11847/zgggws1136689
HAO Yan, CHENG Shi-qi, ZHAO Xiao-tian, . Single and combined effect of vinyl chloride monomer and high-fat diet on gut microbiota in mice[J]. Chinese Journal of Public Health, 2022, 38(11): 1455-1459. doi: 10.11847/zgggws1136689
Citation: HAO Yan, CHENG Shi-qi, ZHAO Xiao-tian, . Single and combined effect of vinyl chloride monomer and high-fat diet on gut microbiota in mice[J]. Chinese Journal of Public Health, 2022, 38(11): 1455-1459. doi: 10.11847/zgggws1136689

氯乙烯染毒、高脂饮食及联合作用对小鼠肠道菌群影响

doi: 10.11847/zgggws1136689
基金项目: 国家自然科学基金(81773405);山西省留学基金(2016 – 056)
详细信息
    作者简介:

    郝岩(1995 – ),男,河北邯郸人,硕士在读,研究方向:职业毒理学

    通讯作者:

    仇玉兰,E-mail:ylqiu@sxmu.edu.cn

  • 中图分类号: R 114

Single and combined effect of vinyl chloride monomer and high-fat diet on gut microbiota in mice

  • 摘要:   目的  探讨氯乙烯(VCM)染毒、高脂饮食及联合作用对小鼠肠道菌群的影响。  方法   SPF级C57BL/6J小鼠6周龄雄性20只,分为对照组、高脂组、VCM组(800 mg/m3)、氯乙烯联合高脂组(简称联合组)。高脂饮食和VCM染毒处理13周,取粪便做16S rDNA测序,分析肠道微生物的改变。  结果  高脂饮食及VCM染毒后,改变了肠道菌群的丰度及群落结构。门水平上,处理组的厚壁菌门(Firmicutes)与拟杆菌门(Bacteroidetes)比值均高于对照组;属水平上,各组肠道微生物聚集程度不同。样本复杂度分析中,高脂组物种数低于对照组和联合组(P<0.05);高脂组香农指数(Shannon)低于其他3个组(P<0.05);多样本比较分析中,各组菌群群落组成存在差异。  结论   氯乙烯染毒、高脂饮食及联合作用导致小鼠肠道微生物失调,两者联合主要改变肠道微生物属水平丰度。
  • 图  1  物种丰度柱形图(门)

    图  2  属水平物种丰度聚类热图

    图  3  α多样性指数组间差异

    注:a P<0.05;aa P<0.01;aaa P<0.01。

    图  4  β多样性指数组间差异

    注:A:PCoA 分析; B:PCA分析。

    表  1  各组中优势菌群的比例改变

    组别FirmicutesBacteroidetesF/B
    对照组0.437680.401881.08907
    高脂组0.713470.133805.33222
    VCM组0.695000.206383.36760
    联合组0.571100.279592.04259
    下载: 导出CSV
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出版历程
  • 收稿日期:  2021-08-27
  • 网络出版日期:  2022-11-24
  • 刊出日期:  2022-11-30

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