Single and combined effect of vinyl chloride monomer and high-fat diet on gut microbiota in mice
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摘要:
目的 探讨氯乙烯（VCM）染毒、高脂饮食及联合作用对小鼠肠道菌群的影响。 方法 SPF级C57BL/6J小鼠6周龄雄性20只，分为对照组、高脂组、VCM组（800 mg/m3）、氯乙烯联合高脂组（简称联合组）。高脂饮食和VCM染毒处理13周，取粪便做16S rDNA测序，分析肠道微生物的改变。 结果 高脂饮食及VCM染毒后，改变了肠道菌群的丰度及群落结构。门水平上，处理组的厚壁菌门(Firmicutes)与拟杆菌门(Bacteroidetes)比值均高于对照组；属水平上，各组肠道微生物聚集程度不同。样本复杂度分析中，高脂组物种数低于对照组和联合组（P＜0.05）；高脂组香农指数(Shannon)低于其他3个组（P＜0.05）；多样本比较分析中，各组菌群群落组成存在差异。 结论 氯乙烯染毒、高脂饮食及联合作用导致小鼠肠道微生物失调，两者联合主要改变肠道微生物属水平丰度。 Abstract:Objective To investigate the impact of vinyl chloride monomer (VCM) alone or in combination with high-fat diet on gut microbe in mice. Methods Twenty 6-week-old male specific pathogen-free C57BL/6J mice were divided into four groups: control, high-fat diet (40% of fat), VCM (static inhalation of 2 hours at dosage of 800 mg/m3, 5 times per week continuously for 13 weeks), and VCM plus high-fat diet. By the end of treatments, fecal samples were collected from the mice for 16S rDNA sequence analysis to examine alterations of the mice′s gut microbe. Results The abundance and community structure of gut microbe changed in the mice with high-fat diet and VCM exposure. In terms of phylum, the ratio of Firmicutes to Bacteroidetes of the mice exposed to VCM and high-fat diet were higher than those of control mice. At genus level, the degree of gut microbes aggregation was different among the four groups. Alpha diversity analysis revealed that the observed gut species of the mice with high-fat diet were inferior to those of the mice of control and VCM plus high-fat diet group (both P < 0.05); the Shannon index for the mice with high-fat diet was significantly lower than that for the mice of other three groups (all P < 0.05). Beta diversity analysis indicated that there were differences in community composition of gut microbes among the four groups. Conclusion Exposure to VCM and high-fat diet, alone or combined, could lead to gut microbial imbalance in mice and the combined exposure mainly affect the abundance of gut microbial genera. -
Key words:
- vinyl chloride monomer /
- high-fat diet /
- gut microbiota /
- 16S rDNA
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表 1 各组中优势菌群的比例改变
组别 Firmicutes Bacteroidetes F/B 对照组 0.43768 0.40188 1.08907 高脂组 0.71347 0.13380 5.33222 VCM组 0.69500 0.20638 3.36760 联合组 0.57110 0.27959 2.04259 
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