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疑似霍乱疫情的实验室快速诊断及分子溯源

熊长辉 杨梦 徐晓倩 王鹏 李小英 唐松

熊长辉, 杨梦, 徐晓倩, 王鹏, 李小英, 唐松. 疑似霍乱疫情的实验室快速诊断及分子溯源[J]. 中国公共卫生, 2022, 38(8): 1062-1065. doi: 10.11847/zgggws1136896
引用本文: 熊长辉, 杨梦, 徐晓倩, 王鹏, 李小英, 唐松. 疑似霍乱疫情的实验室快速诊断及分子溯源[J]. 中国公共卫生, 2022, 38(8): 1062-1065. doi: 10.11847/zgggws1136896
XIONG Chang-hui, YANG Meng, XU Xiao-qian, . Rapid pathogen detection and molecular tracing: a laboratory analysis on a cholera epidemic[J]. Chinese Journal of Public Health, 2022, 38(8): 1062-1065. doi: 10.11847/zgggws1136896
Citation: XIONG Chang-hui, YANG Meng, XU Xiao-qian, . Rapid pathogen detection and molecular tracing: a laboratory analysis on a cholera epidemic[J]. Chinese Journal of Public Health, 2022, 38(8): 1062-1065. doi: 10.11847/zgggws1136896

疑似霍乱疫情的实验室快速诊断及分子溯源

doi: 10.11847/zgggws1136896
基金项目: 江西省科技厅项目基金(20202BBGL73045)
详细信息
    作者简介:

    熊长辉(1982 – ),男,江西南昌人,副主任检验技师,硕士,主要从事病原微生物及分子生物学检验

    通讯作者:

    唐松,E-mail:xch524@126.com

  • 中图分类号: R 516.5

Rapid pathogen detection and molecular tracing: a laboratory analysis on a cholera epidemic

  • 摘要:   目的   对一起疑似霍乱疫情患者进行实验室快速诊断、病原分离、毒力基因检测及溯源分析。  方法  2018年10月21日,对某境外旅行团8名有疑似霍乱疫情腹泻症状人员及13名共同暴露者共采集的21份样本,利用FilmArray多病原快速检测平台和荧光PCR进行快速检测,采用VITEK-2全自动微生物鉴定仪对分离培养出的不同菌落进行生化鉴定,采用荧光PCR检测分离鉴定病原菌毒力基因情况,采用脉冲场凝胶电泳(PFGE)对分离鉴定出的病原菌进行溯源分析。  结果  采集的21份样本中有11份样本检出副溶血弧菌特异性核酸片段且分离到副溶血弧菌,其余10份样本未检出其他病原菌特异性核酸片段且未分离到病原菌。11株副溶血弧菌经PFGE分析提示引起这次疫情的是来源于同一克隆株副溶血弧菌。  结论  实验室多种检测方法的有机结合对霍乱、副溶血弧菌等感染引腹泻疫情病例的实验室快速诊断、病原鉴定及疫情溯源具有重要意义。
  • 图  1  菌株PFGE图谱

    注:M:沙门菌标准菌株H9812经Xba I酶切;1~11:样本经Sfi I酶切。

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出版历程
  • 收稿日期:  2021-09-14
  • 网络出版日期:  2022-07-29
  • 刊出日期:  2022-08-31

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