野生鸟类趾爪携带细菌多样性及其潜在致病性种群分析

姜林; 邓巍; 张淑霞; 杨晓燕

doi:10.11847/zgggws1137091

野生鸟类趾爪携带细菌多样性及其潜在致病性种群分析

doi: 10.11847/zgggws1137091

姜林¹,
邓巍¹,
张淑霞^{1, 2},
杨晓燕^{1, 2, 3, ,}

1.
大理大学东喜玛拉雅研究院，云南 671003
2.
中国三江并流区域生物多样性协同创新中心
3.
三江并流区域生物多样性保护与利用云南省创新团队

基金项目: 第二次青藏高原综合科学考察研究资助（2019QZKK0402）；云南省教育厅科学研究基金（2021Y390）

详细信息

作者简介:
姜林（1995 – ），女，山东济宁人，硕士，研究方向：微生物生态学

通讯作者:
杨晓燕，E-mail：yangxy@eastern-himalaya.cn

中图分类号: Q 938.1
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- 文章访问数: 184
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- 被引次数: 0
出版历程
- 接收日期: 2021-10-09
- 网络出版日期: 2022-12-24
- 刊出日期: 2023-01-31

Species diversity and potential pathogenicity of bacteria carried by wild birds' claws in Yunnan province

1.
Institute of Eastern-Himalaya Biodiversity Research, Dali University, Dali, Yunnan Province 671003, China

摘要

摘要: 目的初步调查野生鸟类趾爪携带细菌的多样性及潜在致病性种群，从生态学角度明确开展野生鸟类携带致病性细菌监测的必要性。方法采集野生鸟类趾爪样品12份，采用基于扩增子序列变体（amplicon sequence variants，ASV）的高通量测序技术分析不同鸟类趾爪携带的细菌多样性及致病性细菌的组成。结果野生鸟类趾爪可携带其生境中的优势细菌，不同鸟类携带的细菌及组成相似度与鸟类生活习性相关。在属和种水平，野生鸟类携带的致病菌总占比均较高，分别为81.25 %和75.00 %，其中，林鸟携带有更多种类的致病细菌；水鸟携带的致病菌除少量为植物病原菌外，其他均为对人致病的细菌。结论野生鸟类趾爪会携带其生境中的致病细菌，对不同类型野生鸟类携带的病原微生物的监测需要纳入公共卫生部门的日常监测范围；同时野生鸟类趾爪可作为重点样品采集部位。
- 野生鸟类 /
- 公共卫生 /
- 高通量测序技术 /
- 传染病 /
- 致病性细菌
Abstract: Objective To investigate species diversity and potential pathogenicity of bacteria carried by wild birds’ claws preliminarily for clarifying the necessity of monitoring pathogenic bacteria carried by wild birds. Methods Toe/claw swab specimens were collected from 12 kinds of wild forest/aquatic birds for isolation of bacteria. Amplicon sequence variants (ASV)-based high-throughput sequencing was used to analyze bacterial species composition and pathogenic bacteria isolated. Results A large number of bacteria dominant in the wild birds’ habitats were detected and the bacterial spectrum and its similarity for the bacteria detected in the wild birds of different species were associated with the birds’ living habitats. Of all the detected bacteria at genus and species levels, 81.25% and 75.00% were pathogenic bacteria, with more kinds of pathogenic bacteria detected in the forest birds and all as human pathogenic bacteria except for a small amount of plant pathogenic bacteria in the aquatic birds. Conclusion Wild birds’ toes/claws could carry pathogenic bacteria from their habitats and the detection of pathogenic microorganisms carried by different types of wild birds needs to be included in the scope of routine monitoring for public health.
- wild birds /
- public health /
- high-throughput sequencing technology /
- infectious disease /
- pathogenic bacteria

HTML全文

图 1 Alpha多样性稀疏曲线

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图 2 不同鸟类细菌门水平群落组成

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图 3 不同鸟类细菌属水平物种群落组成

下载: 全尺寸图片幻灯片

图 4 ASVs水平不同鸟类细菌的PCoA分析

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表 1 不同鸟类所携带细菌群落Alpha多样性指数

物种	ASV数	Chao指数	Ace指数	Shannon指数	Simpson指数
中白鹭	380	380.26	381.39	5.492	88.31 %
绿翅短脚鹎	541	541.49	545.33	6.764	93.74 %
灰眶雀鹛	488	490.62	494.74	7.270	98.43 %
小仙鹟	865	866.11	869.27	7.811	98.85 %
绿背山雀	808	808.78	851.05	7.239	98.04 %
注：物种名称和排列顺序依照《中国鸟类分类与分布名录（第三版）》^[22]

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表 2 种水平不同鸟类携带细菌的分类

细菌种属	中白鹭	绿翅短脚鹎	灰眶雀鹛	小仙鹟	绿背山雀
直肠弯曲菌	0.001475	0.012894	0.002830	0.005685	0.001523
亚弧弯曲菌	0.665078	0.063649	0.069958	0.047545	0.064213
婴儿链球菌	0.004425	0.176406	0.175601	0.174677	0.091624
血肠球菌	0.002269	0.029904	0.019337	0.022739	0.076396
产气荚膜梭菌	0.050488	0.023320	0.011162	0.014729	0.016751
巴氏梭菌	0.015317	0.004664	0.004716	0.005426	0.005838
双酶梭菌	0.051169	0.019479	0.011791	0.011111	0.011675
索氏梭菌	0.029272	0.010974	0.006917	0.008269	0.010660
梭状杆菌	0.035398	0.009053	0.006446	0.008010	0.007868
类志贺邻单胞菌	0.055934	0.033196	0.007389	0.010853	0.006599
毛绒厌氧杆菌	0.004425	0.015364	0.024210	0.044703	0.042386
痤疮丙酸杆菌	0.001929	0.027984	0.004559	0.012145	0.007868
惰性凝聚杆菌	0.002383	0.068861	0.004087	0.010853	0.006091
粘质沙雷菌	0.005446	0.035665	0.009590	0.021705	0.004061
寡养单胞菌	0.001361	0.010425	0.003301	0.002842	0.003553
泡囊短波单胞菌	0.001702	0.007407	0.002044	0.003618	0.004822
立克次氏体	0.001702	0.002195	0.002515	0.001809	0.005076
生黃瘤胃球菌	0.000567	0.003841	0.001100	0.002067	0.005076
约氏不动杆菌	0.006467	0.009602	0.003301	0.002842	0.004061
普氏栖粪杆菌	0.013388	0.006584	0.001729	0.016021	0.002538
索失鲸杆菌	0.038462	0.020576	0.005659	0.015245	0.012183
乳酸杆菌	0.002156	0.024143	0.034586	0.032558	0.016497
分支涅瓦菌	0.001702	0.007956	0.004245	0.006718	0.001269
甲基杆菌	0.000000	0.010700	0.005502	0.006202	0.003807
微黄色奈瑟氏菌	0.000000	0.006859	0.004402	0.002842	0.003046
Rahnella aquatilis	0.000000	0.013169	0.003144	0.001550	0.003807
粘滑罗斯菌	0.000000	0.034568	0.228423	0.005168	0.276396
副流感嗜血杆菌	0.000000	0.171742	0.016350	0.057364	0.040863
殊异韦荣菌	0.000000	0.057064	0.122779	0.214212	0.100254
小韦荣球菌	0.000000	0.035665	0.013363	0.052455	0.029188
Bulleidia moorei	0.000000	0.007407	0.035215	0.020413	0.022843
粪产碱菌	0.000000	0.010700	0.001415	0.008010	0.002030
牙内卟啉单胞菌	0.000000	0.002195	0.013677	0.008010	0.005330
有害新月形单胞菌	0.000000	0.005761	0.002987	0.006718	0.000000
柯氏棒状杆菌	0.000000	0.008230	0.001100	0.004393	0.001777
变黑普氏菌	0.000000	0.002469	0.011476	0.008269	0.008376
产黑素普雷沃菌	0.000000	0.006584	0.118849	0.119380	0.090355
中间普氏菌	0.000000	0.002743	0.004245	0.002842	0.003299
冻水玫瑰单胞菌	0.000908	0.000000	0.000000	0.000000	0.000000
Roseomonas frigidaquae	0.000454	0.000000	0.000000	0.000000	0.000000
罗伊氏乳杆菌	0.003631	0.000000	0.000000	0.000000	0.000000
Methylibium petroleiphilum	0.001248	0.000000	0.000000	0.000000	0.000000
Reyranella massiliensis	0.000794	0.000000	0.000000	0.000000	0.000000
卵形拟杆菌	0.000454	0.000000	0.000000	0.000000	0.000000

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