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KIR2DL4及其配体HLA-G基因多态性与HCV易感性和慢性化关系

冯泽沛 陈琼 张津玮 黄鹏 尹荣 曾添 贾林娜 喻荣彬 张云 岳明

冯泽沛, 陈琼, 张津玮, 黄鹏, 尹荣, 曾添, 贾林娜, 喻荣彬, 张云, 岳明. KIR2DL4及其配体HLA-G基因多态性与HCV易感性和慢性化关系[J]. 中国公共卫生. doi: 10.11847/zgggws1138229
引用本文: 冯泽沛, 陈琼, 张津玮, 黄鹏, 尹荣, 曾添, 贾林娜, 喻荣彬, 张云, 岳明. KIR2DL4及其配体HLA-G基因多态性与HCV易感性和慢性化关系[J]. 中国公共卫生. doi: 10.11847/zgggws1138229
FENG Ze-pei, CHEN Qiong, ZHANG Jin-wei, . Association of genetic variants of KIR2DL4 and its ligand HLA-G gene with HCV susceptibility and chronicity[J]. Chinese Journal of Public Health. doi: 10.11847/zgggws1138229
Citation: FENG Ze-pei, CHEN Qiong, ZHANG Jin-wei, . Association of genetic variants of KIR2DL4 and its ligand HLA-G gene with HCV susceptibility and chronicity[J]. Chinese Journal of Public Health. doi: 10.11847/zgggws1138229

KIR2DL4及其配体HLA-G基因多态性与HCV易感性和慢性化关系

doi: 10.11847/zgggws1138229
基金项目: 国家自然科学基金(81773499);江苏省优秀青年基金(BK20190106);云南省自然科学基金重点项目(2019FA005);新发突发呼吸系统传染病临床研究中心(HS2020002)
详细信息
    作者简介:

    冯泽沛(1997 – ),男,湖北十堰人,硕士在读,研究方向:传染病流行病学

    通讯作者:

    张云,E-mail:zhangyunvip@126.com

    岳明,E-mail:yueming@njmu.edu.cn

  • 中图分类号: R 183

Association of genetic variants of KIR2DL4 and its ligand HLA-G gene with HCV susceptibility and chronicity

  • 摘要:   目的  探讨KIR2DL4基因rs649216位点及其配体HLA-G基因rs1063320位点单核苷酸多态性(SNP)与丙型肝炎病毒(HCV)易感性和慢性化的关联性,为HCV感染预防筛查和诊断提供理论依据。  方法  于2011年10月 — 2015年12月采用整群抽样方法抽取江苏省南京市公安局强制隔离戒毒所和宜兴市强制戒毒所收治的1121例吸毒者及江苏省9家医院的733例肾透析者共1 854例18~80岁未接受过抗HCV治疗(包括干扰素和直接抗病毒药物)且随访 ≥ 6个月的HCV感染高危人群进行问卷调查和HCV抗体检测,将抗 – HCV和HCV RNA均为阴性的1341例作为阴性组、抗 – HCV阳性且HCV RNA阴性的301例作为自限清除组、抗 – HCV和HCV RNA均为阳性的212例作为持续感染组,采用TaqMan探针实时荧光定量聚合酶链式反应(PCR)进行基因分型,并应用多因素logistic回归模型分析KIR2DL4基因rs649216位点及其配体HLA-G基因rs1063320位点基因多态性与HCV易感性和慢性化的关系。  结果  阴性组KIR2DL4 rs649216位点CC、CT和TT型基因型携带者分别占69.15 %、28.15 %和2.69 %,自限清除组分别占73.90 %、23.05 %和3.05 %,持续感染组分别占62.25 %、33.82 %和3.92 %;阴性组HLA-G rs1063320位点GG、GC和CC型基因型携带者分别占32.89 %、51.25 %和15.86 %,自限清除组分别占34.29 %、45.36 %和20.36 %,持续感染组分别占33.15 %、47.28 %和19.57 %。在校正了性别、年龄、谷丙转氨酶(ALT)值是否异常、谷草转氨酶(AST)值是否异常和感染途径后,多因素非条件logistic回归分析结果显示,与携带KIR2DL4 rs649216位点CC基因型的个体相比,携带KIR2DL4 rs649216位点CT/TT基因型的个体HCV慢性化风险更高(共显性模型:OR = 1.682,95 % CI = 1.109~2.551,P = 0.014;显性模型:OR = 1.671,95 % CI = 1.121~2.493,P = 0.012);与携带KIR2DL4 rs649216位点C等位基因的个体相比,携带KIR2DL4 rs649216位点T等位基因的个体HCV慢性化风险更高(相加模型:OR = 1.508,95 % CI = 1.070~2.125,P = 0.019);与携带HLA-G rs1063320位点GG + GC基因型的个体相比,携带HLA-G rs1063320位点CC基因型的个体感染HCV风险更高(隐性模型:OR = 1.361,95 % CI = 1.018~1.819,P = 0.037)。  结论  KIR2DL4基因rs649216位点与HCV慢性化有关,其配体HLA-G rs1063320位点基因多态性与HCV易感性有关。
  • 表  1  KIR2DL4 rs649216位点和HLA-G rs1063320位点基因多态性与HCV易感性和慢性化关系多因素非条件logistic回归分析

    多态性位点基因型阴性组自限清除组持续感染组自限清除组 + 持续感染组vs.阴性组持续感染组vs.自限清除组
    n%n%n%P OR 95 % CIP OR 95 % CI
    rs649216 CC 899 69.15 218 73.90 127 62.25 1.000 1.000
    CT 366 28.15 68 23.05 69 33.82 0.355 0.890 0.695~1.140 0.014 1.682 1.109~2.551
    TT 35 2.69 9 3.05 8 3.92 0.439 1.283 0.683~2.411 0.382 1.587 0.564~4.465
    显性模型 0.504 0.922 0.727~1.170 0.012 1.671 1.121~2.493
    隐性模型 0.377 1.326 0.709~2.482 0.543 1.376 0.492~3.843
    相加模型 0.759 0.968 0.787~1.191 0.019 1.508 1.070~2.125
    rs1063320 GG 396 32.89 96 34.29 61 33.15 1.000 1.000
    GC 617 51.25 127 45.36 87 47.28 0.694 0.949 0.733~1.229 0.894 1.031 0.659~1.613
    CC 191 15.86 57 20.36 36 19.57 0.098 1.319 0.950~1.833 0.617 0.868 0.498~1.512
    显性模型 0.764 1.038 0.814~1.323 0.917 0.978 0.643~1.486
    隐性模型 0.037 1.361 1.018~1.819 0.522 0.853 0.523~1.390
    相加模型 0.179 1.119 0.950~1.319 0.669 0.942 0.718~1.237
      注:a 调整了性别、年龄、ALT值是否异常、AST值是否异常和感染途径等混杂因素。
    下载: 导出CSV
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  • 收稿日期:  2022-02-15
  • 网络出版日期:  2023-01-10

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