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夜间光暴露小鼠肝脏蛋白质组学分析

Differential protein expressions in liver of mice exposed to light at night: a proteomics analysis

  • 摘要:
      目的  筛选分析夜间光暴露小鼠肝脏中差异表达蛋白及其生物学意义。
      方法  选择健康雄性C57BL/6J小鼠随机分为夜间光暴露组和对照组,每组8只。夜间光暴露组光照/黑暗周期为24 h/0 h,对照组为12 h/12 h,连续10 d。应用串联质谱标签(TMT)技术寻找并鉴定差异表达蛋白质,对差异蛋白进行GO富集分析和KEGG信号通路分析。
      结果  共鉴定383种差异表达蛋白质,其中215种蛋白质表达水平上调,168种蛋白质表达水平下调。GO功能富集分析和KEGG信号通路分析表明差异表达蛋白质主要分布在胞质部分,分子功能以蛋白结合为主,主要参与蛋白质和脂质等生物代谢过程,作用途径涉及氧化磷酸化信号通路、肿瘤坏死因子(TNF)信号通路、维甲酸诱导基因I(RIG-I)样受体信号通路等。线粒体细胞色素C氧化酶亚基3(MT-CO3)、粒体Fo复合体亚基F2(ATP5J2)、E3泛素连接酶(ITCH)、核因子κB p65亚基(NF-κB p65)、核因子κB激酶抑制剂β亚基(IKBKB)等蛋白分子可能在其中起到关键作用。
      结论  根据差异表达蛋白及其作用分析,夜间光暴露可能有致肥胖、癌症的危险。

     

    Abstract:
      Objective  To screen and analyze differentially expressed proteins and their biological significance in the liver of mice exposed to light at night.
      Methods  Totally 16 healthy male C57BL/6J mice were randomly divided into an experimental group (n = 8) exposed to light 24 hours a day continuously for 10 days and a control group (n = 8) with lighting 12 hours a day also for 10 consecutive days. Tandem mass tag (TMT) technology was applied to detect differentially expressed proteins in liver tissues of the mice. The differential protein expressions were analyzed using Gene Ontology (GO) enrichment analysis and Kyoto Encyclopedia of Genes and Genomes (KEGG) signaling pathway analysis.
      Results  A total of 383 differentially expressed proteins were identified, of which 215 were expressed at an up-regulated level and 168 proteins were expressed at a down-regulated level. GO enrichment analysis and KEGG signaling pathway analysis revealed that the differentially expressed proteins were mainly distributed in the cytoplasm, and their major molecular functions were protein binding, mainly involved in protein and lipid biological metabolic processes, and their pathways involved in signaling pathways of oxidative phosphorylation, tumor necrosis factor (TNF), and retinoic acid-inducible gene-I (RIG-I)-like receptor. Protein molecules such as mitochondrial cytochrome C oxidase subunit 3 (MT-CO3), mitochondrial Fo complex subunit F2 (ATP5J2), Itchy E3 ubiquitin protein ligase (ITCH), nuclear factor kappa B p65 subunit (NF-κB p65), and inhibitor of nuclear factor Kappa-B kinase subunit Beta (IKBKB) may play a key role.
      Conclusion  In terms of differentially expressed proteins and their functions in liver tissue, light exposure at night may lead to obesity and cancer in mice.

     

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