Efficiency of dual test of SARS-CoV-2 antigen and nucleic acid among freight truck drivers and workers passing through expressway toll gates
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摘要:
目的 对高速卡口货运司乘人员新型冠状病毒抗原与核酸双检测措施效果评估。 方法 对2022年3月20日 — 4月17日在宁波市北仑区8个高速卡口48734名有浙江省外旅居史货运的司乘人员进行新型冠状病毒抗原与核酸双检测,并对检测结果进行描述性统计分析。 结果 共检出9例Omicron变异株感染者,其中确诊病例6例(均为轻型),无症状感染者3例,未发现阳性感染者续发病例;抗原检测试剂的灵敏度和特异度分别为55.5 %(5/9)和99.9 %(48717/48725),阳性预测值和阴性预测值分别为38.5 %(5/13)和99.9 %(48717/48721);阳性感染者中抗原阳性组ORF1ab和N基因Ct值M(P25,P75)分别为19.0(17.1,21.5)、19.0(16.6,22.5),抗原阴性组Ct值均显著高于抗原阳性组(P < 0.05)。 结论 抗原检测试剂灵敏度较低但特异度高。建议在高速卡口对货运司乘人员实施抗原与核酸双检测策略,既保障物流畅通,又有效降低输入性病例引起本土疫情风险。 -
关键词:
- 新型冠状病毒 /
- Omicron变异株 /
- 抗原检测 /
- 核酸检测
Abstract:Objective To evaluate the efficiency of dual test of severe acute respiratory disease coronavirus 2 (SARS-CoV-2) antigen and nucleic acid among freight truck drivers and workers passing through expressway toll gates. Methods Dual tests of SARS-CoV-2 antigen and nucleic acid were conducted among 48 734 freight truck drivers and workers passing through 8 expressway toll gates in Ningbo city from provinces other than Zhejiang province during the period of March 20 – April 17, 2022. The test results were statistically analyzed. Results Totally 9 infections of SARS-CoV-2 Omicron variant were detected; of which, 6 were diagnosed clinically as mild coronavirus disease 2019 (COVID-19) case and 3 as asymptomatic infection. No subsequent COVID-19 case was confirmed. For the SARS-CoV-2 antigen test, the sensitivity and specificity were 55.5% (5/9) and 99.9% (48 717/48 725); the positive and negative predictive value were 38.5% (5/13) and 99.9% (48 717/48 721), respectively. For multiplex fluorescence PCR test of SARS-CoV-2 nucleic acid among the participants with positive SARS-CoV-2 antigen result, the Ct values of ORF1ab and N gene M (25th percentile, 75th percentile) were 19.0 (17.1, 21.5) and 19.0 (16.6, 22.5); while, the Ct values of the participants with negative result was significantly higher than those of the participants with positive SARS-CoV-2 antigen result (P < 0.05). Conclusion In this study, the sensitivity of SARS-CoV-2 antigen test is low but the specificity of the test is high for the detection of SARS-CoV-2 infection. The results suggest that dual test of SARS-CoV-2 antigen and nucleic acid should be implemented to reduce the risk of imported case-induced local COVID-19 epidemic. -
Key words:
- COVID-19 /
- Omicron variant /
- antigen detection /
- nucleic acid detection
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表 1 抗原与核酸检测结果比较
抗原检测结果 核酸检测结果 合计 阳性 阴性 阳性 5 8 13 阴性 4 48717 48721 合计 9 48725 48734 表 2 新冠病毒核酸阳性者中抗原阳性组与抗原阴性组Ct值比较[M(P25,P75)]
抗原检测 N基因 ORF1ab 阳性(n = 5) 19.0(16.6,22.5) 19.0(17.1,21.5) 阴性(n = 4) 31.0(27.1,34.6) 27.1(23.7,30.9) -
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