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基于下一代测序的污水中人星状病毒分子流行病学研究

赵云 纪峰 林小娟 刘尧 徐爱强 陶泽新 温红玲

赵云, 纪峰, 林小娟, 刘尧, 徐爱强, 陶泽新, 温红玲. 基于下一代测序的污水中人星状病毒分子流行病学研究[J]. 中国公共卫生, 2023, 39(11): 1475-1479. doi: 10.11847/zgggws1139435
引用本文: 赵云, 纪峰, 林小娟, 刘尧, 徐爱强, 陶泽新, 温红玲. 基于下一代测序的污水中人星状病毒分子流行病学研究[J]. 中国公共卫生, 2023, 39(11): 1475-1479. doi: 10.11847/zgggws1139435
ZHAO Yun, JI Feng, LIN Xiaojuan, LIU Yao, XU Aiqiang, TAO Zexin, WEN Hongling. Diversity of human astroviruses in untreated domestic sewage: a next generation sequencing analysis in Jining city, Shandong province[J]. Chinese Journal of Public Health, 2023, 39(11): 1475-1479. doi: 10.11847/zgggws1139435
Citation: ZHAO Yun, JI Feng, LIN Xiaojuan, LIU Yao, XU Aiqiang, TAO Zexin, WEN Hongling. Diversity of human astroviruses in untreated domestic sewage: a next generation sequencing analysis in Jining city, Shandong province[J]. Chinese Journal of Public Health, 2023, 39(11): 1475-1479. doi: 10.11847/zgggws1139435

基于下一代测序的污水中人星状病毒分子流行病学研究

doi: 10.11847/zgggws1139435
基金项目: 国家自然科学基金面上项目(81573209);山东省自然科学基金面上项目(ZR2020MH337);泰山学者青年专家计划项目(tsqn202103187)
详细信息
    作者简介:

    赵云(1994 – ),硕士,研究方向:人星状病毒环境污水监测

    通信作者:

    陶泽新,E-mail:sdcdctzx@163.com

    温红玲,E-mail:whling@sdu.edu.cn

Diversity of human astroviruses in untreated domestic sewage: a next generation sequencing analysis in Jining city, Shandong province

More Information
  • 摘要:   目的  了解生活污水中人星状病毒(HAstV)的型别分布及遗传多样性。  方法  2018年1月 — 2019年6月在山东省济宁市按季度采集6份生活污水样本,通过阴离子膜吸附洗脱法进行浓缩后,提取核酸,进行ORF2完整编码区的RT-PCR扩增及扩增产物下一代测序,对产生的干净数据使用CLC Genomics Workbench 12.0 软件de novo 组装后,进行基因定型、同源性比较和系统发生学分析。  结果   检测地区生活污水中HAstV的检出率为100%,共获得HAstV序列43条,分属9种基因型,分别为HAstV-1、HAstV-2、HAstV-3、HAstV-4、HAstV-5等经典型和MLB1、VA1、VA2、VA3等新型HAstV,其中HAstV-5(47.53%)占比最高;基于ORF2完整编码区的系统发生学显示山东株与国内外其他参考株在一些分支上聚集在一起,表明不同型别在世界不同地区的传播。  结论   本研究证实了基于NGS的环境监测有助于提高对HAstV遗传多样性的认识。
  • 图  1  济宁污水样本中HAstV各基因型读长构成季度分布

    Figure  1.  Proportion of HAstV genotype reads for six untreated sewage samples quarterly collected in Jining city - 2018 - 2016

    图  2  人星状病毒ORF2区系统进化树

    注:病毒基因序列按照毒株编号(GenBank序列号)顺序表示。▲山东株序列

    Figure  2.  ORF2 region-based phylogenetic tree for human astrovirus isolated in untreated sewage samples collected in Jining city, Shandong province

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出版历程
  • 接收日期:  2022-06-13
  • 录用日期:  2023-10-25
  • 修回日期:  2023-06-20
  • 网络出版日期:  2023-11-28
  • 刊出日期:  2023-11-01

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