Abstract: Objective To analyse the results of virological surveillance from 1997 to 1999 of acute flaccid paralysis associated with non-polio enterovirus isolation in Hebei Province.Method To use method of tissue cultivation and virus isolation.Results The evaluation demonst rated that during the period of 19971999 47 strains of poliovirus were isolated,they were vaccine virus;isolation of NPEV was 80 strains acounting for 63% of AFP cases.There were coxsackie A 9、5 type of coxsackie B and 10 type of ECHO viruses in the isolation of NPEV.Analysis on the date,there existed peak from June to August in the NPEV infected.The group of under 5 years old was easily infected by NPEV occuping 77.5%,it was 56% under 2 years old.The male was easily infected.The 13.3% persons infected coxsakie B and 22.2% persons infected ECHO remained paralysis after being ill for 60 days.Conclusion The data indicated that,as a group,AFP cases with NPEV isolates are generally unlikely to be confused with poliomyelitis.