Abstract: ObjectiveTo study the two mutation of the pfmdrl gene.MethodUsing the novel Allele-specific PCR and Restriction fragment length polymorphism(RFLP)detected mutation of the pfmdr1 ^Asn86^Tyr and ^Asp1246^Tyr associated with chloro quine resistance in Yunnan to explore an agreement between Chloroquine resistance phenotype and the point mutation.ResultsTen chloroquine resistance isolates was not examed the point mutation of the pfmdr1 Asn 86,while 9 isolates were detected the point mutation(A to T)of the pfmdr1 86^Tyr.However,all samples were not examined mutation of the pfmdr1 Asp ^Asp1246^Tyr.The incomplete agreement found between chloroquine resistance phenotype and Pfmdrl(86^Tyr)mutation supporting the view that other genetic factors as well as pfmdr1 may be involved in chloroquine resistance.The Asp 1246^Tyr seemed to be absent in Yunnan.