Abstract: Objective To investigate the relationship between TTV and primary liver carcinoma.Methods TTV-DNA was detected by nested PCR among three groups of population including 48 cases of primary liver carcinoma(case group),46 cases of non liver malignant tumor(control group 1)and 58 cases of general population(control group 2).Results The positive rate of TTV DNA was 29.17%(14/48)in case group,13.04%(6/46)in control group 1 and 13.79%(8/58)in control group 2 respectively.The differ ence between case group and control group 1 to 2 was closely remar kable(χ²=3.646,P=0.056;χ²=3.774,P= 0.052,respectively).Conclusion TTV was existed in patients with primary liver carcinoma.The difference between case group and control group or nonliver malignant tumor group was closely remarkable.TTV may be anetiology factor for primary liver carcinoma.