Abstract: Objective To study the reproductive toxicity of Thioglycolic Acid(TGA) in mice.Methods The effects of TGA on oocyte maturation and in vitro fertilization(IVF) in mice were studied by the method in vitro culture and IVF in mice oocyte.Results The results showed that TGA could inhibit the germinal vesicle breakdown(GVBD) of mouse oocyte in vitro culture,but had no impact on GVBD in vivo.TGA could also inhibit the extruding of first polar bady and affect the quality and viability of mouse oocytes and reduce the fertilization rate of IVF and the oocytes number which were stimulated through superovulation.Conclusion TGA might be hazardous to the meiotic maturation of mouse oocyte and might reduce the fertility of oocyte.That meant TGA had a reproductive toxicity to female mice to some extent.