Abstract: Objective In order to study the immune effects of two doses and types of genetic engineering hepatitis B vaccines,using RIA to detect the antigen and antibody of hepatitis B in the serum.Methods Before reinforce immunization,the serum antibody protective rate of 195 first grade primary school pupils who received three doses basic immunization by blood vaccines are 70%.Results One month after reinforce immunization,the serum antibody protective rate of two groups which reinforce by different 5μg dose vaccinces are 90% and 98.31%,and the serum antibody protective rate of 10μg dose vaccine group up to 98.48%.Two doses and types of genetic engineering hepatitis B vaccines all have no particular reaction after reinforce immunization.The immune effects are all good.Conclusion Two doses and types of genetic engineering hepatitis B vaccines all have excellent protective effect and can be used as reinforce immunization after blood vaccines basic immunization.The 10μg dose genetic engineering hepatitis B vaccine is better than 5μg genetic engineering hepatitis B vaccine.The 10μg dose vaccine to protect the risk population should first used.