硒对氧自由基引起大鼠脑线粒体损伤的影响

Effects of Selenium on Oxygen Free Radical Induced Injury of Rat Cerebral Mitochondria

摘要: 目的探讨硒对氧自由基引起的大鼠脑线粒体损伤的影响.方法采用Fe²⁺-半胱氨酸(Cys)为氧自由基生成系统,在体外模仿脑出血或脑外伤引起的氧自由基损伤模型,观测不同浓度(5.0×10^-5～5.0μmol/L)亚硒酸钠对Fe²⁺-Cys致损伤脑线粒体的肿胀程度、膜流动性、MDA含量以及线粒体形态结构的影响.结果低浓度硒(5.0×10^-4～5.0×10-2μmol/L)可明显防止氧自由基对线粒体的损伤,高浓度硒(0.5～5.0μmol/L)则产生毒性作用,二者均至剂量-效应关系.结论低浓度硒有利于延缓衰老和防治某些神经系统疾病.

Abstract: Objective The effects of selenium on oxygen free radical inducing injury of rat cerebral mitochondria were investigated.Methods The oxygen free radicals(OFRs)injured cerebral mitochondria induced by Fe²⁺-Cys in vitro were exposed to 5.0×10^-5-5.0 mol/L.Na₂SeO₃ and the changes of injured mitochondria swelling,membrane fluidity,MDA levels and mitochondria structures were observed.Results Low Se concentration(5.0×10^-4-5.0×10^-2 mol/L)can inhabit the injury of rat cerebral mitochondria induced by oxygen free radicals while high Se concentration(0.5-5.0 mol/L) cause toxicity and both are with a relationship of dose-dependent response.Conclusion Low Se concentration is helpful to delay aging and against nervous disease.