不同施药周期DDVP对德国小蠊酶活性的影响

沈孝兵; 张丽

doi:10.11847/zgggws2003-19-04-12

不同施药周期DDVP对德国小蠊酶活性的影响

沈孝兵,
张丽

Effects of DDVP at different period on enzyme activity in German cockroaches

摘要

摘要:
目的探讨德国小蠊在敌敌畏(DDVP)不同施药周期作用下的酶活性变化规律.
方法采用分光光度法测定德国小蠊乙酰胆碱酯酶、酸性磷酸酯酶、羧酸酯酶及α-乙酸萘脂(1-NA)和β-乙酸萘酯(2-NA)酶的活性, 应用SAS软件进行统计分析.
结果随着施药周期的延长, 乙酰胆碱酯酶酶活性受抑制, 酶活性降低, 随后受抑制程度缓慢降低, 酶活性逐渐上升; 酸性磷酸酯酶的活性呈上升趋势; 羧酸酯酶、1-NA和2-NA酶都存在随施药周期的延长, 酶活性上升后又降低的趋势.
结论乙酰胆碱酯酶是DDVP的主要作用靶部位之一, 受DDVP作用影响最大.酸性磷酸酯酶和1-NA是DDVP的重要代谢酶.

Abstract:
Objective To investigate the effects on acety lcholine esterase, car box yester ase, acid-phosphatase, 1-NA and 2-NA activity in German cockro aches exposed to DDVP at different period.
Methods All enzyme activities were measured by visible spectrophotometry and SAS soft ware was used for statistical analysis.
Results With the prolonging exposed to DDVP, the degree of acetylcholine esterase inhibition increase gradually at the beg inning and decrease lately.The activity of acid-phosphatase raised generally.The change of carboxy esterase, 1-NA and 2-NA activity were in opposition to acetylcholine esterase activity's.
Conclusion A cetylcho line esterase was the main target enzyme of DDV P.Acid-phosphatase and 1-NA were the important metabolic enzymes.

HTML全文

参考文献(13)

施引文献

资源附件(0)