SEA致敏树突状细胞免疫小鼠Th1/Th2细胞因子

沈定文; 罗金萍; 李雍龙

doi:10.11847/zgggws2005-21-09-25

SEA致敏树突状细胞免疫小鼠Th1/Th2细胞因子

Effect of Th1/Th2 cytokines on mice immunized with soluble eeg antigen of Schistosoma japonicum pulsed dendritic cells

摘要

摘要:
目的探讨Th1/Th2细胞因子在日本血吸虫可溶性虫卵抗原(SEA)致敏树突状细胞(DC)免疫小鼠抗感染中的作用.
方法采用SEA致敏DC免疫BALB/c小鼠, ELISA分别检测免疫前、后小鼠血清干扰素(IFN)-γ和白细胞介素-4(IL-4)的含量, 脾细胞培养法检测经刀豆蛋白A(ConA)和SEA刺激后, 小鼠脾细胞分泌IFN-γ和IL-4的水平.
结果 SEA致敏DC免疫后血清IFN-γ的水平无明显变化, 而IL-4的含量明显增加(P < 0.01);经ConA、SEA刺激, SEA致敏DC免疫组脾细胞产生较高水平的IL-4(P < 0.01), 而IFN-γ水平与其他组无明显变化.
结论 SEA致敏DC免疫主要诱导Th2免疫反应, 在抗血吸虫感染保护性免疫中起主要作用.

Abstract:
Objective To explore the effect of Th 1/Th 2 cyto kines in the protective immunity against infection of Schis tosoma japonicum in mice immunized with soluble eeg antigen(SEA)pulsed dendrit ic cells(DC).
Methods BALB/c mice were immunized with SEA pulsed DC.ELISAkits were used to ex amine the lev els of IFN-and IL-4 before and 4 weeks after immunization.By the culture of spleen cells, IFN-and IL-4 after stimulation with SEA were quantified by ELISA kits.
Results The level of IL-4 was increased significantly after immunized with SEA pulsed DC(P < 0.01).There was not obvious change in the level of IFN-.With spleen cells fr om SEA pulsed DC vaccinated mice, high level of IL-4 was pro duced in response to ConA and SEA(P < 0.01), while there was nodifference between group of SEA pulsed DC immunization and control group.
Conclusion SEA pulsed DC could induce Th 2 immune response which might play an important role in the protective immunity against infection of Schistosoma japonicum.

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