甲基汞急性暴露下大鼠脑c-fos mRNA表达

杨义晨; 程金平; 胡卫萱; 王文华; 吴旦; 贾金平

doi:10.11847/zgggws2005-21-09-29

甲基汞急性暴露下大鼠脑c-fos mRNA表达

Effects of methylmarcury acute exposure on c-fos mRNA expression in brain of rat

摘要

摘要:
目的探讨即刻早期基因c fos在甲基汞神经毒性分子机制中的作用.
方法应用RT PCR方法研究大鼠注射甲基汞急性暴露后, 中枢神经系统c fosmRNA表达.
结果甲基汞在剂量为0.05mg/(kg·d)暴露60min和剂量为0.5mg/(kg·d)暴露20min就能够显著诱导大鼠脑即刻早期基因c fos表达; c fosmRNA表达量与汞的浓度没有明显正比关系.
结论甲基汞能够显著诱导大鼠脑c fosmRNA表达, c fosmRNA表达变化在甲基汞神经毒性分子机制中具有重要作用, 即刻早期基因c fos参与了甲基汞对中枢神经系统损害的毒性过程.

Abstract:
Objective To study the role that immediate early expression gene(IEG)c fos played in the neurotox ic mech anism, and the effect of methylmarcury(MMC)on c fos mRNAexpression were invest igated.
Methods RTPCRwas used for c fos mRNAex pression after MM Cacute exposure in rats brain.The control group was physiological saline of 0.9%, the concentration of exposure groups were 0.05, 0.5 and 5 mg/kg respectively, the sampling time were 20, 60, 240 and 1 440 min.
Results The 0.05 mg/kg group induced the ex pression of c fos obviously in rat s brain afterex posed for 60min while the 0.5 mg/kg group induced the expression of c fos clearly in rats brain afterexposed for 20min, and the expressive quantity of c fos mRNAdid notheave direct ratio with the concentration of mer cury.
Conclusion The ex pression of c fos mRNA played an important part in the neurotoxic mechanism of MM C; IEGc fos took part in the process that MM Cimpaired the nerve system.

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