系列水溶性壳聚糖衍生物的抑菌性能研究

杨宇民; 马振祥; 尹继成; 温李楠; 陈冠灿

doi:10.11847/zgggws2005-21-09-32

系列水溶性壳聚糖衍生物的抑菌性能研究

Study on antimicrobial activity of chitosan water soluble derivatives

摘要

摘要:
目的检测壳聚糖水溶性衍生物羧甲基壳聚糖、水溶性低分子壳聚糖、氨基葡萄糖盐酸盐、壳寡糖等对金黄色葡萄球菌的抑菌活性.
方法采用营养肉汤稀释法, 测定壳聚糖衍生物对金黄色葡萄球菌的最小抑菌浓度, 从而评价它们各自的抑菌能力.
结果羧甲基壳聚糖、水溶性低分子壳聚糖、氨基葡萄糖盐酸盐对金黄色葡萄球菌的最小抑菌值分别为10.0, 2.5, 12.5mg/ml(样品溶液pH值均为7.2), 其中水溶性低分子壳聚糖的抑菌能力最强, 而壳寡糖在此条件下, 对金黄色葡萄球菌则没有抑菌性.
结论除壳寡糖外, 大多壳聚糖对金黄色葡萄球菌的生长有不同程度的抑制作用.

Abstract:
Objective To investig ate the antimicrobial activ ity of chitosans water soluble derivatives such as carboxymethyl chitosan, water soluble low molecular weight chitosan, glucosamine hydrochloride, chitooligosaccharde, and their minimal inhibitory concentration(MIC)to the growth of staphylococcus aureus were determined.
Methods Using nutrient broth dilution method, MIC to the growth of staphy lococcus aureus of water soluble chitosan derivates were measured to determine the antimicrobial activity.
Results Under the condition of this experiment(the pHvalue of the nutrient brothe was 7.2), the MICof carbox ymethyl chitosan, water soluble low molecular weight chitosan、glucosamine hydro chloride were 10.0 mg/ml, 2.5 mg/ml, 12.5 mg/ml respectively.
Conclusion These chitosan derivates had some antimicrobial activity to the growthe of staphylo coccus aureus and the water soluble low molecular weight chitosan had the strongest antimicrobial activity, while the chitoo ligosaccharde had no antimicrobial activity to the growth of staphylococcus aureus in this condition.

HTML全文

参考文献(7)

施引文献

资源附件(0)