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黄琼, 李志, 黄建康, 杨杏芬, 黄俊明, 蔡玟. CD69+/NKG2D+比值在保健食品评价中应用[J]. 中国公共卫生, 2005, 21(9): 1095-1096. DOI: 10.11847/zgggws2005-21-09-40
引用本文: 黄琼, 李志, 黄建康, 杨杏芬, 黄俊明, 蔡玟. CD69+/NKG2D+比值在保健食品评价中应用[J]. 中国公共卫生, 2005, 21(9): 1095-1096. DOI: 10.11847/zgggws2005-21-09-40
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HUANG Qiong, LI Zhi, HUANG Jiankang, . Application of CD69+/NKG2D+ in health food evaluation[J]. Chinese Journal of Public Health, 2005, 21(9): 1095-1096. DOI: 10.11847/zgggws2005-21-09-40
Citation: HUANG Qiong, LI Zhi, HUANG Jiankang, . Application of CD69+/NKG2D+ in health food evaluation[J]. Chinese Journal of Public Health, 2005, 21(9): 1095-1096. DOI: 10.11847/zgggws2005-21-09-40
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CD69+/NKG2D+比值在保健食品评价中应用

Application of CD69+/NKG2D+ in health food evaluation

    摘要
  • 摘要:
      目的   研究小鼠外周血自然杀伤细胞(NK)细胞分化抗原CD69+/NKG2D+检测在保健食品免疫调节作用评价中的应用.
      方法   以左旋咪唑25mg(kg·bw)连续灌胃3d设立左旋咪唑阳性对照; 进行小鼠外周血自然杀伤细胞(NK)亚群CD69+/NKG2D+检测和脾NK细胞活性测定试验, 观察0.83, 1.67g/(kg·bw)剂量组蛋白粉对小鼠NK细胞免疫功能的影响.
      结果   与阴性对照组比较, 左旋咪唑对照组和0.83, 1.67g/(kg·bw)剂量组蛋白粉剂量组外周血NK细胞CD69-/NKG2D+、CD69+/NKG2D+比值以及脾NK细胞活性均明显升高.
      结论   外周血NK细胞CD69+/NKG2D+检测与传统的NK细胞免疫检测结果有较好一致性, 且方法敏感性较好, 在保健食品免疫调节功能评价中有较好应用价值.

     

    Abstract:
      Objective   To apply the peripheral blood NKcell CD69+/NKG2D+ in the imunity function evaluation of health food.
      Methods   To build the positive control through treated with levamisole25 mg/(kg bw)by oral feeding for 3d;Peripher al blood NKcell Sub cluster CD69+ /NKG2D+, spleen NKcell activity test were carried out to investigate the ef fects of protein powder0.83, 1.67 mg/(kg·bw)on NKcellular immunity function of mice.
      Results   Compared with the negative control, activity of spleen NKcell and peripheral blood NKcell sub cluster CD69- /NKG2D+ and CD69+ /NKG2D+ of Levamisole group and0.83, 1.67 mg/(kg·bw)group were all significantly increased.
      Conclusion   Levamiso le has en hancingeffects on the NKcellular immunity function of mice, and can be used as the positive control in the imunit y function evaluation of health food; The results of the peripheral blood NKcell CD69+ /NKG2D+ and the traditional test for activity of NKcell have good coherence, and the former method has good susceptiv ity and applicat ion value in the NKcellular immunity function ev aluation of health food.

     

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