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谭晓华, 李冬妹, 狄春红, 杨磊, 何玲, 普雄明. 卡波氏肉瘤患者8型疱疹病毒套式PCR检测[J]. 中国公共卫生, 2005, 21(12): 1414-1415. DOI: 10.11847/zgggws2005-21-12-04
引用本文: 谭晓华, 李冬妹, 狄春红, 杨磊, 何玲, 普雄明. 卡波氏肉瘤患者8型疱疹病毒套式PCR检测[J]. 中国公共卫生, 2005, 21(12): 1414-1415. DOI: 10.11847/zgggws2005-21-12-04
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TAN Xiaohua, LI Dongmei, DI Chunhong, . Detection of human herpesvirus 8 from kaposi's sarcoma by nested-PCR[J]. Chinese Journal of Public Health, 2005, 21(12): 1414-1415. DOI: 10.11847/zgggws2005-21-12-04
Citation: TAN Xiaohua, LI Dongmei, DI Chunhong, . Detection of human herpesvirus 8 from kaposi's sarcoma by nested-PCR[J]. Chinese Journal of Public Health, 2005, 21(12): 1414-1415. DOI: 10.11847/zgggws2005-21-12-04
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卡波氏肉瘤患者8型疱疹病毒套式PCR检测

Detection of human herpesvirus 8 from kaposi's sarcoma by nested-PCR

    摘要
  • 摘要:
      目的   探讨8型疱疹病毒(human herpesvirus 8, HHV 8)与新疆卡波氏肉瘤(kaposi's sarcoma, KS)发病的关系并探索HHV8 DNA的检测方法。
      方法   采用巢式聚合酶链反应(nested-polymerase chain reaction, nested-PCR)方法检测29例新疆KS患者血清、43份石蜡包埋KS组织HHV8 DNA, 并检测68份非KS患者血清HHV8 DNA作为对照。
      结果   KS组阳性率分别为86.21%(25/29)和95.35%(41/43), 2种样本间HHV8 DNA检出率差异无统计学意义(χ2=1.90, P=0.173)。对照组68例非KS患者血清HHV8 DNA阳性率为20.59%(14/68)。KS组与对照组间差异有统计学意义(χ2=72.15, P < 0.001)。
      结论   结果提示HHV8是KS的病原, 同时建立了比较灵敏的nested-PCR检测血清HHV8 DNA的方法。

     

    Abstract:
      Objective   To confirm the relationship between Human herpesvirus 8(HHV8)and the pathogeny of Kaposis sarcoma(KS)in Xinjiang.
      Methods   Nested-PCR was applied to detect HHV8 DNA that extracted from KS serum(or biopsy)and non KS serum.
      Results   HHV 8 DNA was detected in 25 out of 29(86.21%)KS serum, and 41 out of 43 KSbiopsy.The difference between the two type of samples was not significant(χ2=1.90, P=0.173).And in non KS controls, 14 out of 68(20.59%)were positive.The differences were statistically significant between the KS group and non KS group (χ2=72.15, P < 0.001).
      Conclusion   These data imply that HHV 8 is the pathogeny of KS, and a more sensitive method was established to detect HHV 8 DNA from serum by nested-PCR.

     

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