马齿苋的抗低氧作用及其机制研究

岳义田; 董立巍; 李敏; 凌昌全

doi:10.11847/zgggws2005-21-12-14

马齿苋的抗低氧作用及其机制研究

Study of Purslane Herb on anti-hypoxia and its probable mechanism

摘要

摘要:
目的观察不同浓度马齿苋水提液对低氧小鼠的保护作用及其机制。
方法以生理盐水和人参总皂苷为对照, 观察不同浓度马齿苋水提液对密闭低氧条件下小鼠存活时间的影响, 采用生物化学方法测定丙酮酸激酶(PK)和磷酸果糖激酶(PEK)活性, 荧光素酶法测定ATP含量, 定磷法测定Na⁺, K⁺-ATP酶活性, 分光光度法测定乳酸含量, 2, 4二硝基苯肼比色法测定乳酸脱氢酶(LDH)活性。
结果 (1) 0.5, 1.0, 2.0 g/ml 3种浓度的马齿苋水提液分别延长密闭低氧小鼠的生存时间24%, 36.5%和27.4%;(2)1 g/ml马齿苋水提液可明显增强低氧小鼠心、脑细胞PK、PEK活性, 缓解细胞内ATP含量下降幅度, 提高Na⁺, K⁺-ATP、Ca²⁺, Mg²⁺-ATP和LDH等酶的活性。
结论马齿苋水提液可提高小鼠耐低氧能力, 机制之一可能是其促进低氧小鼠无氧酵解关键酶的活性, 进而缓解因低氧引起能量代谢障碍所致的细胞损伤。

Abstract:
Objective To investigate the effects of Purslane Herb aqueous extracts(PHAS)on anti-hypoxia ability of mice.
Methods The survival time to hypoxia of the mice treated with different doses of PHAS and saponins panax ginseng were observed.The activities of Phosphohructokinase(PFK), Pyruvate kinase(PK), Na⁺, K⁺-ATPase, Ca²⁺, Mg²⁺-Atpase and lactate dehydrogenase(LDH), and contents of ATP and lactic acid in the cardiac myocytes and brain celles were tested.
Results As compared with hypoxia model group, three doses of PHAS(0.5, 1.0 and 2.0 ml/d)and saponins panax ginseng prolonged the survival time to hypoxia, and the 1.0 ml/d PHAS had the best effect.In the group of 1.0 ml/d PHAS, the activity of PFK, P K, Na⁺, K⁺-ATPase, Ca²⁺, Mg²⁺-ATPese, and LDH decreased less, and contents of ATP was higher significantly.
Conclusion PHDS can lengthen the survival time of hypooxia stressed mice.One of its mechanisms may be accelerating the activity of the key enaymes in glycolysis.

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