危险品货运站空气污染物对DNA的损伤作用
Study on DNA damage by air pollutants in transport station of chemical dangerous goods
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摘要:目的 探讨危险品货运站空气污染物的DNA损伤作用。方法 应用SOS/umu试验, 大鼠肺Ⅱ型细胞的DNA交联试验和单细胞凝胶电泳试验, 检测危险品货运站空气污染物对DNA的损伤情况。结果 空气污染物在81, 163, 325, 650μg/10μl的剂量范围, 不加S9活化系统的条件下, 污染物显示遗传毒性, 存在剂量-反应关系(r=0.994, P<0.01);加入S9活化系统后, 污染物未显示出遗传毒性。在1.02, 2.03, 4.06, 8.13 mg/ml的剂量范围内, 污染物能引起大鼠肺Ⅱ型细胞DNA产生双链间的交联和单链断裂, 存在剂量-反应关系(r交联=0.981, r断裂=0.987, P<0.01)。结论 危险品货运站空气污染物具有DNA损伤作用。Abstract:Objective To explore the DNA damage induced by air pollutants in a transport station of chemical dangerous goods(TSCDG).Methods SOS/umuassay and the ethidium bromide flurescence assay and the single cell gel electrophoresis assay were applied respectively to detect the DNA damage and DNA cross-links and DNA single strand breaks after exposure to the particle extracts of air pollutants in TSCDG.Results The air pollution of TSCDG at asingle dose of 81, 163, 325, 650 g.The results showed that air pollution in TSCDG induced significant SOS response to TA1535/PSK 1002 strain and exhibited obvious dose-response relationships(r=0.994, P<0.01).It had not showed genotoxicity with liver microsomal metabolic activation.The air pollution of TSCDG at a single dose of 1.02, 2.03, 4.06, 8.13 mg/ml.DNA cross-links and DNA single strand breaks in rat type lung cells were found after exposed and the dose-response relationships were existed between formation of DNA cross-links or DNA single strand breaks on rat type lung cells and exposed concentrations of the extracts(rcross-links=0 981, rbreaks=0.987, P<0.01).Conclusion The air pollutants in TSCDG may result in DNA damage.
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