煤灰粉致大鼠肺损害及纤维化因子的释放
Study on lung injury and fibrogenic cytokine production induced by coal fly ash in rats
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摘要:目的 观察煤灰粉对大鼠肺组织损害及纤维化因子释放的影响, 探讨其致尘肺作用机制。方法 收集某热电厂煤灰粉, 以石英尘作为阳性对照, 生理盐水作为阴性对照, 对大鼠进行一次性气管内注入染尘。染尘后10d处死动物, 立即行支气管肺泡灌洗, 检测支气管肺泡灌洗液(BAIF)中乳酸脱氢酶(LDH)、总蛋白和磷脂的含量, 免疫组织化学检测肺组织肿瘤坏死因子-α(TNF-α)、转化生长因子-β1(TGF-β1)和转化生长因子-α(TGF-α)的表达。结果 煤灰粉引起大鼠肺BALF中LDH、总蛋白和磷脂的含量分别为(6.18±0.24)×103u/L, (0.67±0.02)g/L和(0.48±0.02)mmol/L, 比对照组分别为(3.62±0.21)×103u/L, (0.28±0, 02)g/L和(0.23±0.01)mmol/L明显增加(P < 0.05)。对照织大鼠肺组织不表达TNF-α和TGF-α, 煤灰粉引起大鼠肺组织TNF-α和TGF-β1表达增强(P < 0.05), 每高倍镜视野阳性细胞数分别为(30.33±1.86)和(40.02±4.20)。结论 煤灰粉引起一定程度的肺组织损害及纤维化因子TNF-α和TGF-β1的释放可能与其致尘肺作用有关。Abstract:Objective To observe the effects of coal fly ash on lung injury and fibrogenic cytokine production in rats and elucidate the pathogenesis of the pneumoconiosis caused by the coal fly ash.Methods The rats were inoculated intratracheally with coal fly ash from a thermal power station.The quartz was used as a positive dust.Controls were similarly inoculated with physiological saline.All the animals were killed 10 days after dust inoculation.The lungs were immediately lavaged.The cell-free supernatant was separated for examining the contents of lactate dehydrogenase(LDH), the total proteins, and the total phospholipid.The lung sections were placed on slides for immunohistochemistry of tumor necrosis factor-α(TNF-α), transforing growth factor-β(TGF-β1)and transforming growth factor-α(TGF-α).Results Compared with the controls, the contents of LDH, the total proteins and the total phopholipid were significantly elevated by coal fly ash(P < 0.05).The lung of controls showed no TNF-α and TGF-β1 labeling.The increases of TNF-α and-β1 expression in the lung tissue of rats were observed in the coal fly ash group(P < 0.05).Conclusion Lung injury and increased production of fibrogenic cytokine TNF-αand TGF2β1 may play an important role in the pneumoconiosis caused by the coal fly ash.
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