Abstract:
Objective To study the molecular epidemiolo gy of poliov irus isolated from the feces of acute flaccid paralysis cases in Fujian.
Methods The order genetic fragment was amplified by RT-PCR and then sequenced.The result of the virus sequence was given to NCBIGenebank for contr ast,and co nstructed the phylong enetictrees.
Results 5 poliovirus strains isolated in Fujian from 1999 to 2002 years,which were SabinⅡ and SabinⅢ-recombinant strains.The sequence analysis sho wed the identit ies of these 5 poliovirus strains and standerd vaccine was over 99%,ithad seldom difference in nucletide,and was seldom difference from Panzhihua strains.
Conclusion The vaccine-recombinant polioviruses had even been in Fujian from 1999 to 2002,one of the virus strain had already circled in human,and caused two cases.At present,our nation had already been polio-free,we must pay attention to vaccine-derived polioviruses strains when we still use OPV to immunize.The method of molecular epidemiology can provide us support to adopt crrelation strategy to prevent and control polio in time.
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