对苯二酚与杂交中DNA的相互作用

Interaction between hydroquinone and deoxgribonucleic acid in hybridization

摘要:
目的探讨在pH为7.42磷酸盐缓冲溶液中对苯二酚与DNA的相互作用。
方法用循环伏安法、荧光光谱法、紫外光谱法、DNA分子杂交技术和量子化学方法进行检测。
结果在DNA杂交过程中, 对苯二酚与其作用后的荧光光谱, 紫外光谱的吸收峰峰形以及强度改变较大, 循环伏安法氧化峰正向移动, 量化计算结果表明, 对苯二酚位于磷酸基团和碱基近侧。
结论在DNA杂交过程中对苯二酚与其以∏-∏化学键和静电结合为主。

Abstract:
Objective To study the interaction between hydroquionoes and deoxgribonucleic acid in pH 7.42 sodium phosphate buffer solution.
Methods Ultraviole-tvisble spectrophotometry, fluorescence spectrophotometry, cylic voltammetry, hybridization and quantum chemistry were used.
Results Peaks and the intension of absorption and fluorescence spectra changed obviously in hybridization, the oxidated peak of cyclic voltammetry shifted to postivie, the quantum chemistry theoretical calculation indicated that the hydroquinone closed to the phosphate groups and base groups in DNA.
Conclusion The electrostatic attraction and П-П banding between hydroquinones and deoxgribonucleic acid are the major modes for the interaction in hybridization.