氯化镉对人肝癌细胞SMMC-7721抑制作用

Inhibition effect of cadmium chloride on human hepatocellular carcinoma cells SMMC-7721

摘要:
目的研究氯化镉(CdCl ₂)对人肝癌细胞(SMMC-7721)的抑制作用并探讨其作用机制。
方法应用生长曲线法、四甲基偶氮噻唑蓝法(MTT)、流式细胞术(FCM)检测CdCl ₂对SMMC-7721的生长抑制及对细胞周期的影响。
结果 CdCl ₂对肝癌细胞SMMC-7721有明显的生长抑制作用, 且存在剂量-效应关系; 影响细胞周期进程并出现G₀/G₁期阻滞。
结论 CdCl ₂能有效抑制肝癌细胞株的生长, 其抑制作用可能与影响细胞周期进程有关。

Abstract:
Objective To study the inhibition effect of cadmium chloride(CdCl ₂)on detected by growth curve and methyl thiazolyl tetrazolium(MTT)assay, and cell cycle was determined by flow cytometry(FCM).
Results CdCl ₂ showed inhibition effect on the proliferation of SMM C-7721 in dose-dependent manner.Cell cycle was arrested in G ₀/G ₁ phase.
Conclusion CdCl ₂ possesses growth inhibition effect on hepatocellular carcinoma cells and the mechanism may be related to cell cycle arrested.