不同来源产志贺毒素大肠埃希菌分布特征

Distribution and characterization of Shiga toxin-producing E.coli from different samlpes

摘要:
目的探讨不同标本中产志贺样毒素大肠埃希菌(STEC)的分布特征。
方法采集动物粪便、肉类食品和排污口污泥样品, 常规分离大肠埃希菌, 血清学分型, PCR鉴定产志贺样毒素(stxl, stx2)菌株。
结果 293份标本中鉴定出8株STEC, 1株为产志贺毒素O157:H7型, 2株为不产志贺毒素O157:H7型, 5株为产志贺毒素非O157:H7型。
结论 STEC存在于不同来源的标本中, 菌株表型与毒力因子存在一定差异。

Abstract:
Objective To explore the distribution and character ization of Shig a tox in-producing E.coli from animal feces, meat food and contaminatemud.
Methods Routine method was used to isolate E.coli, strains of Shiga tox in1-2 producing were detected by PCR assay; and types were analyzed by ser ological technique.
Results Of all samples, 8 isolates were identified as Shiga toxin-producing E.coli, one strain was identified as EHEC O157:H7, two strains as EHEC O157:H7 with no Shiga tox in and five strains as no-EHEC O157:H7 with producing Shiga toxin.
Conclusion ST ECcould be separated from different samples, and there was slightly distance between types and pathogenic factors.