钾离子外流在小鼠精子获能中作用

龚伟; 程洁; 张习春; 王昌松; 肖杭

doi:10.11847/zgggws2006-22-04-49

钾离子外流在小鼠精子获能中作用

Effects of potassium ion efflux on mouse sperm in vitro capacitaion in mice

摘要

摘要:
目的观察钾离子外流在小鼠精子体外获能的作用及机制。
方法以金霉素(chlortetracycline, CTC)荧光染色后涂片观察小鼠精子体外获能率, 进一步应用膜片钳技术在小鼠生精细胞膜上记录延迟整流钾离子电流(IDRK)。
结果终浓度为50 nmol/L的钾离子通道载体缬氨霉素处理小鼠精子, 其B型精子发生率(%)与对照组相比显著增加(n=10, P < 0.05);50 nmol/L缬氨霉素显著增大小鼠粗线期精母细胞延迟整流钾离子电流, 上调其Ⅰ-Ⅴ曲线。
结论缬氨霉素通过激活延迟整流钾通道, 增加胞内钾离子的外流, 促发小鼠精子体外获能。

Abstract:
Objective To observe the correlation between the mouse sperm capacitaion state and K⁺ channel and its mechanism.
Methods Mice sperm in vitro capacitation was assessed by the B pattern of staining by chlortetracycline (CTC).Then the delayed rectifier K⁺ currents(Ⅰ_DRK)in mouse pachy tene spermatocyte were recorded using whole-cell patch clamp technique.
Results When spermatozo a were cultured in valinomycin(Val, a potassium ionophore)at the concentration of 50 nmol/L, the capacitation was increased significantly(n=10, P < 0.05).50 nmol/L Val increased the amplitude of IDRK currents significantly, up-regulated the-Ⅳ curve of IDRK current.
Conclusion Valinomcin promotes potassiumion efflux by activation of delayed rectifier potassium channels in mouse pachytene spermatocyte, and then mediated hyperpolarization itself appears capable of inducing sperm capacitation.

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